Comparative Transcriptome Analysis of Genes Involved in GA-GID1-DELLA Regulatory Module in Symbiotic and Asymbiotic Seed Germination of Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae)
| Autor: | Si-Si Liu, Juan Chen, Xu Zeng, Shu-Chao Li, Zhixia Meng, Shun-Xing Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Orchidaceae
Anoectochilus roxburghii transcriptome seed germination symbiotic asymbiotic GA-GID1-DELLA Germination Biology Genes Plant Catalysis Article lcsh:Chemistry Inorganic Chemistry Transcriptome Symbiosis Gene Expression Regulation Plant Mycorrhizae Botany Databases Genetic Gene Regulatory Networks Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Gene Spectroscopy Plant Proteins Gene Expression Profiling Organic Chemistry food and beverages Molecular Sequence Annotation General Medicine biology.organism_classification Computer Science Applications Transcriptome Sequencing Gene expression profiling Gene Ontology lcsh:Biology (General) lcsh:QD1-999 Seeds |
| Zdroj: | International Journal of Molecular Sciences; Volume 16; Issue 12; Pages: 30190-30203 International Journal of Molecular Sciences Volume 16 Issue 12 Pages 30190-30203 International Journal of Molecular Sciences, Vol 16, Iss 12, Pp 30190-30203 (2015) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms161226224 |
| Popis: | Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae) is an endangered medicinal plant in China, also called “King Medicine”. Due to lacking of sufficient nutrients in dust-like seeds, orchid species depend on mycorrhizal fungi for seed germination in the wild. As part of a conservation plan for the species, research on seed germination is necessary. However, the molecular mechanism of seed germination and underlying orchid-fungus interactions during symbiotic germination are poorly understood. In this study, Illumina HiSeq 4000 transcriptome sequencing was performed to generate a substantial sequence dataset of germinating A. roxburghii seed. A mean of 44,214,845 clean reads were obtained from each sample. 173,781 unigenes with a mean length of 653 nt were obtained. A total of 51,514 (29.64%) sequences were annotated, among these, 49 unigenes encoding proteins involved in GA-GID1-DELLA regulatory module, including 31 unigenes involved in GA metabolism pathway, 5 unigenes encoding GID1, 11 unigenes for DELLA and 2 unigenes for GID2. A total of 11,881 genes showed significant differential expression in the symbiotic germinating seed sample compared with the asymbiotic germinating seed sample, of which six were involved in the GA-GID1-DELLA regulatory module, and suggested that they might be induced or suppressed by fungi. These results will help us understand better the molecular mechanism of orchid seed germination and orchid-fungus symbiosis. |
| Databáze: | OpenAIRE |
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