Effect of several analogs of 2,4,6-triphenyldioxane-1,3 on CYP2B induction in mouse liver
| Autor: | Nikolai Slynko, Yuliya Kazakova, Vladimir O. Pustylnyak, Lyudmila F. Gulyaeva, Andrei A. Yarushkin |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Chromatin Immunoprecipitation Blotting Western Biology Toxicology Polymerase Chain Reaction Dioxanes Mice Cytochrome P-450 Enzyme System Constitutive androstane receptor Animals Inducer Binding site Gene DNA Primers Regulation of gene expression Base Sequence Dose-Response Relationship Drug General Medicine Molecular biology Mice Inbred C57BL Biochemistry Mrna level Liver Enzyme Induction Electrophoresis Polyacrylamide Gel Target gene |
| Zdroj: | Chemico-biological interactions. 194(2-3) |
| ISSN: | 1872-7786 |
| Popis: | 2,4,6-Triphenyldioxane-1,3 (TPD) is a highly effective inducer of CYP2В in rats, but not in mice. Several analogs of TPD were synthesized. All substituents were entered into the same position of TPD (R = H, cisTPD and transTPD; R = N(CH3)2, transpDMA; R = NO2, transpNO2; R = F, transpF; R = OCH3, transpMeO). The purpose of the present study was to investigate the effect of TPD analogs on CYP2B induction in mouse livers. Among the six test compounds, four (R = –N(CH3)2, –NO2, –F, –OCH3) demonstrated a dose-dependent induction of mouse CYP2B. To further characterize the compounds, we determined ED50s using sigmoidal dose–response curves. The dose–response study has shown that all active compounds have similar potencies to induce CYP2B in mouse livers. Western-blot analysis and multiplex RT-PCR have shown that the increase of CYP2B activity in mouse liver is related to the high content of CYP2B proteins and paralleled the increase of cyp2b10 mRNA level. ChIP results have demonstrated that the transcriptional enhancement of cyp2b10 gene in response to compounds is accompanied by the increased recruitment of the constitutive androstane receptor (CAR) to its specific binding site (PBREM) on the target gene. Thus, minor structural changes in TPD cause dramatic changes in its ability to induce mouse CYP2B, and it is likely several TPD analogs act by activation of mouse CAR. |
| Databáze: | OpenAIRE |
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