Molecular detection of Blastocystis sp. subtype 14 in the Yezo sika deer (Cervus nippon yesoensis) in Hokkaido, Japan
Autor: | Mami Koketsu, Yu-ki Morishita, Takahiro Shirozu, Shinya Fukumoto |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Cervus General Veterinary biology Deer 030231 tropical medicine Ribosomal rna gene Zoology 030108 mycology & parasitology biology.organism_classification Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Japan parasitic diseases Small subunit Blastocystis Blastocystis sp Animals Parasitology Colonization Cattle Feces Phylogeny Cervus nippon yesoensis |
Zdroj: | Veterinary parasitology, regional studies and reports. 25 |
ISSN: | 2405-9390 |
Popis: | This study describes the first report of Blastocystis sp. colonization in the sika deer (Cervus nippon) in Japan and in other animals in Hokkaido, Japan. Blastocystis sp. is one of the most widespread intestinal protist in a wide range of animals. Blastocystis sp. isolated from mammalian and avian species have been classified into 17 subtypes (STs). Some of the STs are zoonotic. The aim of this study was to evaluate Blastocystis sp. colonization in the Yezo sika deer (Cervus nippon yesoensis) in Hokkaido, Japan. The Yezo sika deer are currently overabundant and they are expanding their habitat to humans and livestock. A total of 132 deer fecal samples were subjected for molecular detection of Blastocystis sp. Of these, 60 (45.5%) samples were positive using PCR, which targets the small subunit ribosomal RNA gene sequence. All Blastocystis sp. DNA sequences from the Yezo sika deer were genotyped into ST14, which were originally reported in cattle. These findings indicate that the current public health risks of Blastocystis sp. from the Yezo sika deer is low, although more detailed future analysis is required. |
Databáze: | OpenAIRE |
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