Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA
| Autor: | Roberto Gambino, Francesco Ramirez, Dan Kacian, Winston Salser, Arthur Bank, Gary V. Paddock, Howard C. Heindell, Philip Whitcome, Raymond Poon |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
DNA polymerase
chemistry.chemical_compound Ribonucleases Complementary DNA Animals Globin Amino Acid Sequence RNA Messenger Multidisciplinary biology Base Sequence Phosphoric Diester Hydrolases RNA-Directed DNA Polymerase Nucleic acid sequence RNA DNA Templates Genetic Alkaline Phosphatase Molecular biology Reverse transcriptase Globins Biochemistry chemistry biology.protein Rabbits Biological Sciences: Biochemistry Phosphorus Radioisotopes |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 71(9) |
| ISSN: | 0027-8424 |
| Popis: | Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of 32 P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T 1 and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the α or β globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants. |
| Databáze: | OpenAIRE |
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