Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats
| Autor: | Quinten R Ducarmon, Daniele Mandrioli, Romy D. Zwittink, Jean Michel Panoff, Laura Falcioni, Maxime Teixeira, Anna Caldwell, Robin Mesnage, Francesca Mazzacuva, Fiorella Belpoggi, Caroline Amiel, John M. Halket, Michael Antoniou |
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| Rok vydání: | 2021 |
| Předmět: |
animal structures
Health Toxicology and Mutagenesis Glycine 010501 environmental sciences Gut flora 01 natural sciences Microbiology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Sprague dawley rats Metabolome Animals Shikimate pathway 030212 general & internal medicine Health implications 0105 earth and related environmental sciences Acinetobacter integumentary system biology Herbicides Research fungi Public Health Environmental and Occupational Health food and beverages biology.organism_classification Gastrointestinal Microbiome Rats Blood chemistry Glyphosate Metagenomics Shotgun metagenomics |
| Zdroj: | Environmental Health Perspectives, 129(1). US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE Environmental Health Perspectives |
| ISSN: | 1552-9924 0091-6765 |
| Popis: | BACKGROUND: There is intense debate on whether glyphosate can inhibit the shikimate pathway of gastrointestinal microorganisms, with potential health implications.OBJECTIVES: We tested whether glyphosate or its representative EU herbicide formulation Roundup MON 52276 affects the rat gut microbiome.METHODS: We combined cecal microbiome shotgun metagenomics with serum and cecum metabolomics to assess the effects of glyphosate [0.5, 50, 175 mg=kg body weight oBW thorn per day] or MON 52276 at the same glyphosate-equivalent doses, in a 90-d toxicity test in rats.RESULTS: Glyphosate and MON 52276 treatment resulted in ceca accumulation of shikimic acid and 3-dehydroshikimic acid, suggesting inhibition of 5-enolpyruvylshikimate-3-phosphate synthase of the shikimate pathway in the gut microbiome. Cysteinylglycine, c-glutamylglutamine, and valylglycine levels were elevated in the cecal microbiome following glyphosate and MON 52276 treatments. Altered cecum metabolites were not differentially expressed in serum, suggesting that the glyphosate and MON 52276 impact on gut microbial metabolism had limited consequences on physiological biochemistry. Serum metabolites differentially expressed with glyphosate treatment were associated with nicotinamide, branched-chain amino acid, methionine, cysteine, and taurine metabolism, indicative of a response to oxidative stress. MON 52276 had similar, but more pronounced, effects than glyphosate on the serum metabolome. Shotgun metagenomics of the cecum showed that treatment with glyphosate and MON 52276 resulted in higher levels of Eggerthella spp., Shinella zoogleoides, Acinetobacter johnsonii, and Akkermansia muciniphila. Shinella zoogleoides was higher only with MON 52276 exposure. In vitro culture assays with Lacticaseibacillus rhamnosus strains showed that Roundup GT plus inhibited growth at concentrations at which MON 52276 and glyphosate had no effect.DISCUSSION: Our study highlights the power of multi-omics approaches to investigate the toxic effects of pesticides. Multi-omics revealed that glyphosate and MON 52276 inhibited the shikimate pathway in the rat gut microbiome. Our findings could be used to develop biomarkers for epidemiologi cal studies aimed at evaluating the effects of glyphosate herbicides on humans. https://doi.org/10.1289/EHP6990 |
| Databáze: | OpenAIRE |
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