Respiratory Syncytial Virus Glycoprotein Expression in Human and Simian Cell Lines
| Autor: | A. C. R. Samson, E. G. Routledge, M. M. Willcocks, R. Scott, Geoffrey L. Toms |
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| Rok vydání: | 1986 |
| Předmět: |
Paramyxoviridae
medicine.drug_class Immunoprecipitation Biology Monoclonal antibody Virus Epitope Cell Line Epitopes Viral Proteins Viral Envelope Proteins Virology Chlorocebus aethiops medicine Animals Humans Antigens Viral Glycoproteins chemistry.chemical_classification Membrane Glycoproteins biology.organism_classification Molecular biology Fusion protein Respiratory Syncytial Viruses chemistry Cell culture Glycoprotein Viral Fusion Proteins |
| Zdroj: | Journal of General Virology. 67:2059-2064 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-67-9-2059 |
| Popis: | Summary Glycoproteins synthesized in both human (HeLa and HEp-2) and simian (Vero and BS-C-1) cell lines following infection with two different strains of respiratory syncytial virus (A2 and Long) were analysed by SDS-PAGE following immunoprecipitation with monoclonal antibodies. Minor virus strain-dependent differences in the large glycoprotein, G, and the fusion protein polypeptides F1 and F2 were observed together with minor cell line-dependent differences in the size of the F2 polypeptide. Major quantities of two glycoproteins, termed Ga (50K) and Gb (45K), were detected in A2 strain-, and to a lesser extent in Long strain-, infected simian cells. These proteins were also present in infected human cells, but in much reduced amounts. Immunoprecipitation with anti-G monoclonal antibodies demonstrated that Ga and Gb shared different epitopes with G. |
| Databáze: | OpenAIRE |
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