Epstein-Barr virus-associated post-transplant lympho-proliferative disease of donor origin in liver transplant recipients
| Autor: | Chiara Menin, Rosa Maria Iemmolo, Emma D'Andrea, Alvise Maffei Faccioli, Mario Strazzabosco, Alessandro Poletti, Roberto Merenda, Annarosa Del Mistro, Laura Bonaldi, Daniele Neri, M. Montagna, Giorgio Enrico Gerunda, Barbara Corneo |
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| Přispěvatelé: | Strazzabosco, M, Corneo, B, Iemmolo, R, Menin, C, Gerunda, G, Bonaldi, L, Merenda, R, Neri, D, Poletti, A, Montagna, M, Del Mistro, A, Faccioli, A, D'Andrea, E |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Male
Herpesvirus 4 Human Pathology medicine.medical_specialty medicine.medical_treatment In situ hybridization Liver transplantation medicine.disease_cause Herpesviridae Virus Cell Line MED/12 - GASTROENTEROLOGIA medicine liver transpantation epstein-barr virus Humans Gammaherpesvirinae Southern blot B-Lymphocytes Hepatology biology business.industry Herpesviridae Infections Middle Aged biology.organism_classification Epstein–Barr virus Lymphoproliferative Disorders Tissue Donors Liver Transplantation Transplantation Tumor Virus Infections Immunology Tomography X-Ray Computed business Microsatellite Repeats |
| Popis: | Background/Aims: Post-transplant lymphoproliferative disease, a potential complication of solid organ transplantation, occurs in about 3% of orthotopic liver transplant recipients. We report the genetic and virological characterization of two cases of post-transplant lymphoproliferative disease that occurred early (4 and 6 months) after orthotopic liver transplant as large-cell non-Hodgkin's lymphomas located at the hepatic hilum. Methods: Lymphomatous tissues were analyzed for clonality and presence of Epstein-Barr virus (EBV) sequences by Southern blot, polymerase chain reaction, and in situ hybridization techniques. Results: The tumors in both cases were sustained by a clonal proliferation of B lymphocytes containing type A EBV DNA. Moreover, in situ hybridization with a digoxigenin-labeled EBV-specific probe evidenced a strong nuclear signal in most of the neoplastic cells. DNA microsatellite analysis at three different loci detected alleles of donor origin in both tumor samples, suggesting that the neoplastic B cells were of donor origin. Conclusions: EBV-infected donor B lymphocytes might be responsible for intragraft post-transplant lymphoproliferative disease in orthotopic liver transplant recipients. As 20 to 30% of post-transplant lymphomas involve the graft itself, donor-derived post-transplant lymphoproliferative disease might be more frequent than present appreciated. Prospective studies are needed to assess its real incidence and identify possible risk factors. |
| Databáze: | OpenAIRE |
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