Antibacterial, antioxidant, and topical anti-inflammatory activities of Bergia ammannioides: A wound-healing plant

Autor: Zeinab A. Kandil, Mouchira A. Choucry, Shahira M. Ezzat
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
Antioxidant
medicine.medical_treatment
Ethyl acetate
Anti-Inflammatory Agents
Pharmaceutical Science
Wounds
Penetrating
Microbial Sensitivity Tests
Administration
Cutaneous
Antioxidants
Ointments
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Picrates
Drug Discovery
medicine
Organic chemistry
Animals
Skin
Pharmacology
Wound Healing
Plants
Medicinal
biology
Traditional medicine
Plant Extracts
Biphenyl Compounds
Biological activity
General Medicine
Elatinaceae
Plant Components
Aerial
biology.organism_classification
Anti-Bacterial Agents
030104 developmental biology
Complementary and alternative medicine
chemistry
030220 oncology & carcinogenesis
Cycloartenol
Molecular Medicine
Bergia ammannioides
Topical anti-inflammatory
Wound healing
Zdroj: Pharmaceutical biology. 54(2)
ISSN: 1744-5116
Popis: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for the treatment of wounds in India, there is a scarcity of scientific data supporting this use.The objective of this study is to assess wound-healing potentiality of the plant, to study pharmacological activities that may contribute in eliminating wound complications, and to investigate the biologically active fractions.The ethanolic extract (EtOH) of the aerial parts was fractionated to obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions. EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically investigated to isolate their constituents.Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62, and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the strongest anti-inflammatory (64.5% potency relative to Voltaren®) and antibacterial activity (MIC = 104 μg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant activity against DPPH, ABTS(•+), and super oxide radical with an IC50 value of 10.25 ± 0.01, 66.09 ± 0.76, and 167.33 ± 0.91 µg/ml, respectively. β-Sitosterol, lupeol, cyclolaudenol, and cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-α-l-rhamnoside, and quercetin-3-O-α-l-rhamnoside were isolated from EtFr.Our study presents scientific evidence for the efficacy of B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and cycloartenol from Bergia.
Databáze: OpenAIRE
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