Simultaneous voltammetry detection of dopamine and uric acid in human serum and urine with a poly(procaterol hydrochloride) modified glassy carbon electrode
| Autor: | Jinwei Su, Yejian Han, Dexian Kong, Chun-Hua Lu, Lili Jiang, Qizhao Zhuang, Lanping Xu, Yuwu Chi, Zeming Wang |
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| Rok vydání: | 2018 |
| Předmět: |
Analyte
Polymers Dopamine Biosensing Techniques 02 engineering and technology 010402 general chemistry Electrochemistry 01 natural sciences Analytical Chemistry Procaterol Humans Electrodes Voltammetry Detection limit Chemistry Electrochemical Techniques 021001 nanoscience & nanotechnology Carbon Uric Acid 0104 chemical sciences Membrane Electrode Glass Cyclic voltammetry 0210 nano-technology Selectivity Nuclear chemistry |
| Zdroj: | Talanta. 185:203-212 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2018.03.078 |
| Popis: | In the present study, procaterol hydrochloride (ProH) was successfully electropolymerized onto a glass carbon electrode (GCE) with simply cyclic voltammetry scans to construct a poly(procaterol hydrochloride) (p-ProH) membrane modified electrode. Compared with the bare GCE, much higher oxidation peak current responses and better peak potentials separation could be obtained for the simultaneous oxidation of dopamine (DA) and uric acid (UA), owning to the excellent electrocatalytic ability of the p-ProH membrane. And it's based on that a square wave voltammetry (SWV) method was developed to selective and simultaneous measurement of DA and UA. Under the optimum conditions, the linear dependence of oxidation peak current on analyte concentrations were found to be 1.0–100 μmol/L and 2–100 μmol/L, giving detection limits of 0.3 μmol/L and 0.5 μmol/L for DA and UA, separately. The as prepared modified electrode shows simplicity in construction with the merits of good reproducibility, high stability, passable selectivity and nice sensitivity. Finally, the proposed p-ProH membrane modified electrode was successfully devoted to the detection of DA and UA in biological fluids such as human serum and urine with acceptable results. |
| Databáze: | OpenAIRE |
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