Determination of mexiletine in biological fluids by high-performance liquid chromatography
| Autor: | Michael Wilfling, Henning Breithaupt |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Detection limit
Time Factors Chromatography Propylamines Extraction (chemistry) Mexiletine General Chemistry Urine High-performance liquid chromatography chemistry.chemical_compound Column chromatography Pharmacokinetics chemistry Reference Values medicine Humans Diethyl ether Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 230:97-105 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(00)81434-6 |
| Popis: | A high-performance liquid chromatographic method for the determination of the anti-arrhythmic drug mexiletine in human plasma, urine, and cerebrospinal fluid is described. Following extraction with diethyl ether, mexiletine and the internal standard 4-methylmexiletine were derivatized with 2,4-dinitrofluorobenzene. Analyses were performed using an alternating on-column enrichment technique on small Perisorb RP-2 30-40 micrometers pre-columns with pre-column backflushing for direct injection onto the analytical column of Spherisorb ODS 5 micrometers. Complete separation from endogenous constituents of plasma, urine or cerebrospinal fluid was achieved by isocratic reversed-phase ion-pair chromatography with 1-heptanesulfonic acid (0.005 M; PIC B7)-acetonitrile-tetrahydrofuran (42:48:10, v/v) as eluent. Interferences from other drugs were not observed. The limit of detection was 10 ng/ml (C.V. 6.5%). Day-to-day coefficients of variation were below 9%. The applicability of this rapid method for pharmacokinetic studies and clinical routine is demonstrated. |
| Databáze: | OpenAIRE |
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