In vitro mineral nutrition of Curcuma longa L. affects production of volatile compounds in rhizomes after transfer to the greenhouse
| Autor: | Alan Janbey, Mary H. Grace, Rabia Fawzi El-Hawaz, Jeffrey Adelberg, Mary Ann Lila |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Potassium Compounds Bioreactor Germacrone Plant Science In Vitro Techniques engineering.material 01 natural sciences Gas Chromatography-Mass Spectrometry Phosphates 03 medical and health sciences chemistry.chemical_compound Bioreactors Curcuma Nutrient Fed-batch technique lcsh:Botany Magnesium Curcuma longa rhizome Food science Fertilizers Minerals Volatile Organic Compounds Nitrates biology Correction Phosphate biology.organism_classification lcsh:QK1-989 Rhizome 030104 developmental biology chemistry Phytochemical engineering Calcium Fertilizer GC-MS Gas chromatography–mass spectrometry Response surface methods Sesquiterpenes 010606 plant biology & botany |
| Zdroj: | BMC Plant Biology, Vol 18, Iss 1, Pp 1-13 (2018) BMC Plant Biology |
| ISSN: | 1471-2229 |
| Popis: | Background Turmeric is a rich source of bioactive compounds useful in both medicine and cuisine. Mineral concentrations effects (PO4 3−, Ca2+, Mg2+, and KNO3) were tested during in vitro rhizome development on the ex vitro content of volatile constituents in rhizomes after 6 months in the greenhouse. A response surface method (D-optimal criteria) was repeated in both high and low-input fertilizer treatments. Control plants were grown on Murashige and Skoog (MS) medium, acclimatized in the greenhouse and grown in the field. The volatile constituents were investigated by GC-MS. Results The total content of volatiles was affected by fertilizer treatments, and in vitro treatment with Ca2+ and KNO3; but PO4 3− and Mg2+ had no significant effect. The content was higher in the high-input fertilizer treatments (49.7 ± 9 mg/g DM) with 4 mM Ca2+, 60 mM KNO3 and 5 mM NH4 +, than the low-input fertilizer (26.6 ± 9 mg/g DM), and the MS control (15.28 ± 2.7 mg/g DM; 3 mM Ca2+, 20 mM K+, 39 mM NO3 −, 20 mM NH4 +, 1.25 mM PO4 3−, and 1.5 mM Mg2+). The interaction of Ca2+ with KNO3 affected curcumenol isomer I and II, germacrone, isocurcumenol, and β-elemenone content. Increasing in vitro phosphate concentration to 6.25 mM increased ex vitro neocurdione and methenolone contents. Conclusion These results show that minerals in the in vitro bioreactor medium during rhizome development affected biosynthesis of turmeric volatile components after transfer to the greenhouse six months later. The multi-factor design identified 1) nutrient regulation of specific components within unique phytochemical profile for Curcuma longa L. clone 35–1 and 2) the varied phytochemical profiles were maintained with integrity during the greenhouse growth in high fertility conditions. |
| Databáze: | OpenAIRE |
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