Effect of a Chloroacetyl Modification on the Suppression of Dissociation of a Fluorescent Molecule from Cells for Antigen-Specific Cell Staining

Autor:	Ryosuke Kaneko, Masumi Kawamura, Takeshi Mori, Akihiro Kishimura, Yoshiki Katayama
Rok vydání:	2020
Předmět:	chemistry.chemical_classification Staining and Labeling Acetates Fluorescence Dissociation (chemistry) Analytical Chemistry Staining Hydrophobic effect Enzyme Microscopy Fluorescence Antigen chemistry Covalent bond Hydrolase Tumor Cells Cultured Biophysics Humans K562 Cells Fluorescent Dyes
Zdroj:	Analytical Sciences. 37:529-532
ISSN:	1348-2246 0910-6340
DOI:	10.2116/analsci.20scn03
Popis:	We previously developed a hydrolase-based fluorescence amplification method for antigen-specific cell labelling, in which fluorescent substrates stained cells by a non-covalent hydrophobic interaction. To improve the substrates retention in cells, we examined the effect of a chloroacetyl group modification on the substrate retention. We found that the chloroacetyl group suppressed the dissociation of the substrate after forming a covalent bond with intracellular proteins. However, the slow reaction speed of the chloroacetyl group allowed dissociation for cells in the early stage of the staining reaction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a67f843d84091bb400f3720c327439e3 https://doi.org/10.2116/analsci.20scn03 Zobrazit plný text záznamu Full text from SpringerLink