Regulation by Iron: RNA Rules the Rust
| Autor: | Robert J. Kadner |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
RNA
Untranslated Superoxide Dismutase Iron RNA Oxidation reduction Gene Expression Regulation Bacterial Biology Microbiology Redox Rust Cofactor Repressor Proteins Bacterial protein RNA Bacterial Bacterial Proteins Biochemistry Operon Escherichia coli biology.protein RNA Messenger Guest Commentaries Promoter Regions Genetic Molecular Biology Sulfur |
| Zdroj: | Journal of Bacteriology. 187:6870-6873 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.187.20.6870-6873.2005 |
| Popis: | RyhB is a noncoding RNA regulated by the Fur repressor. It has previously been shown to cause the rapid degradation of a number of mRNAs that encode proteins that utilize iron. Here we examine the effect of ectopic RyhB production on global gene expression by microarray analysis. Many of the previously identified targets were found, as well as other mRNAs encoding iron-binding proteins, bringing the total number of regulated operons to at least 18, encoding 56 genes. The two major operons involved in Fe-S cluster assembly showed different behavior; the isc operon appears to be a direct target of RyhB action, while the suf operon does not. This is consistent with previous findings suggesting that the suf genes but not the isc genes are important for Fe-S cluster synthesis under iron-limiting conditions, presumably for essential iron-binding proteins. In addition, we observed repression of Fur-regulated genes upon RyhB expression, interpreted as due to intracellular iron sparing resulting from reduced synthesis of iron-binding proteins. Our results demonstrate the broad effects of a single noncoding RNA on iron homeostasis. |
| Databáze: | OpenAIRE |
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