Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism
| Autor: | Saradhadevi Varadharaj, Rashmeet K. Reen, Jay L. Zweier, Binata Joddar, Rachel C. Childers, Mahmood Khan, Keith J. Gooch, Michael S. Firstenberg |
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| Rok vydání: | 2014 |
| Předmět: |
inorganic chemicals
Pathology medicine.medical_specialty Intimal hyperplasia lcsh:Medicine Organ culture chemistry.chemical_compound medicine Humans Saphenous Vein lcsh:Science chemistry.chemical_classification Tiron Reactive oxygen species Aldehydes Multidisciplinary Hyperplasia business.industry lcsh:R Anatomy Arteries Free Radical Scavengers Tunica intima medicine.disease Oxygen medicine.anatomical_structure chemistry cardiovascular system 1 2-Dihydroxybenzene-3 5-Disulfonic Acid Disodium Salt lcsh:Q Lipid Peroxidation business Reactive Oxygen Species Tunica Intima Ex vivo Artery circulatory and respiratory physiology Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 3, p e0120301 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Saphenous veins used as arterial grafts are exposed to arterial levels of oxygen partial pressure (pO2), which are much greater than what they experience in their native environment. The object of this study is to determine the impact of exposing human saphenous veins to arterial pO2. Saphenous veins and left internal mammary arteries from consenting patients undergoing coronary artery bypass grafting were cultured ex vivo for 2 weeks in the presence of arterial or venous pO2 using an established organ culture model. Saphenous veins cultured with arterial pO2 developed intimal hyperplasia as evidenced by 2.8-fold greater intimal area and 5.8-fold increase in cell proliferation compared to those freshly isolated. Saphenous veins cultured at venous pO2 or internal mammary arteries cultured at arterial pO2 did not develop intimal hyperplasia. Intimal hyperplasia was accompanied by two markers of elevated reactive oxygen species (ROS): increased dihydroethidium associated fluorescence (4-fold, p |
| Databáze: | OpenAIRE |
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