High level of B19 strain detection in Brazilian cattle semen
| Autor: | Anna Monteiro Correia Lima, Muriell Ribeiro Ganda, A. Ferreira Junior, Jandra Pacheco dos Santos, Mariana Assunção de Souza, M. P. S. Lourencetti, S Miyashiro |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Veterinary medicine 040301 veterinary sciences Brucella Vaccine Brucella abortus Semen Polymerase Chain Reaction Brucellosis Semen collection law.invention 0403 veterinary science Vaccine strain Food Animals law medicine Animals Polymerase chain reaction DNA Primers Strain (chemistry) biology 0402 animal and dairy science 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Antibodies Bacterial 040201 dairy & animal science biology.protein Cattle Animal Science and Zoology Antibody Brazil Bacteria |
| Zdroj: | Tropical Animal Health and Production. 50:433-439 |
| ISSN: | 1573-7438 0049-4747 |
| Popis: | Semen contaminated with microorganisms can disseminate serious diseases including brucellosis. The objectives of this study were to detect Brucella-specific antibodies and Brucella abortus DNA in samples of blood and fresh semen from 100 animals older than 20 months. The samples were collected on farms and in semen collection and processing centers (CCPS). The serum samples were evaluated by Rose Bengal test (RBT). B. abortus DNA was detected by a polymerase chain reaction (PCR) using BAB and IS771 primers. The difference between the vaccine field strain was identified using ery-1, ery-2, and ery-3 primers, using the hemi-nested PCR method. No anti-B. abortus antibodies were detected in the serum samples. Out of the total semen samples, 68% (68/100) presented amplifications of the B. abortus genes. All (68/68) were identified as B19 strain of Brucella abortus vaccine. It was concluded that even bulls that are seronegative for brucellosis can eliminate the bacteria in the semen. The presence in the DNA of the B19 vaccine strain should be investigated for a better understanding of the epidemiological importance of this strain in these animals. |
| Databáze: | OpenAIRE |
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