Further purification of group A streptococcal pyrogenic exotoxin and characterization of the purified toxin
| Autor: | E L Barsumian, Dennis W. Watson, C M Cunningham |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Pyrogens
Streptococcus pyogenes Isoelectric focusing Toxin Immunology Ion chromatography Erythrogenic toxin Biology Chromatography Ion Exchange medicine.disease_cause Ouchterlony double immunodiffusion Microbiology In vitro Structure-Activity Relationship Infectious Diseases Bacterial Proteins Biochemistry medicine Parasitology Hyaluronic Acid Exotoxin Research Article Skin Tests Toxins Biological |
| Zdroj: | Infection and Immunity. 14:767-775 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.14.3.767-775.1976 |
| Popis: | Streptococcal pyrogenic exotoxin (SPE) isolated from culture filtrates of strain NY-5 (type 10), and separated from other extracellular by differential solubility in ethanol and acetate-buffered saline, has previously been shown to exhibit a wide range of biological activities including erythrogenic activity, pyrogenicity, enhancement of susceptibility to endotoxin shock, blockage of the reticuloendothelial system immmunosuppression, and lymphocyte mitogenicity. Toxin prepared in this way was found to consist of hyaluronic acid and several proteins which could be distinguished by thin-layer polyacrylamide isoelectric focusing (IEF), SPE has been further purified by ion exchange chromatography on QAE-Sephadex columns. One of the fractions isolated from QAE-Sephadex, and shown to be a homogenous protein by thin-layer IEF and Ouchterlony with hyperimmune serum, was highly active erythrogenically, pyrogenically, and in enhancing susceptibility to endotoxin. This fraction was identified as exotoxin A. A second, less active fraction identified as SPE B showed similar activities, but differed from the other fraction antigenically and in net charge and molecular weight. These findings indicate that a single highly purified protein can mediate at least three of the biological activities attributed to SPE and NY-5 produces pyrogenic exotoxins A and B in vitro as well as in vivo. |
| Databáze: | OpenAIRE |
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