A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction
| Autor: | G. Ferrini, Salvatore Costanzo, Gerardo Zambrano, Flavia Nastri, Rosaria Cercola, R. Battaglia, Angela Lombardi, Vincenzo Pavone, Marco Mauro, Ornella Maglio |
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| Přispěvatelé: | Maglio, Ornella, Costanzo, Salvatore, Cercola, Rosaria, Zambrano, Gerardo, DI MAURO, Marco, Battaglia, Raffaele, Ferrini, Gianluca, Nastri, Flavia, Pavone, Vincenzo, Lombardi, Angelina |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
medicine.drug_class Population Nanotechnology Biosensing Techniques 02 engineering and technology QCM immunosensor lcsh:Chemical technology Monoclonal antibody 01 natural sciences Biochemistry Article Analytical Chemistry Crystal stem cells Dental pulp stem cells Monolayer medicine lcsh:TP1-1185 Electrical and Electronic Engineering education Instrumentation Immunoassay education.field_of_study 010401 analytical chemistry anti-CD34 antibody self-assembled monolayer dental pulp Self-assembled monolayer Quartz Quartz crystal microbalance 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences stem cell Biotinylation Quartz Crystal Microbalance Techniques Gold 0210 nano-technology |
| Zdroj: | Sensors (Basel) 17 (2017). doi:10.3390/s17122747 info:cnr-pdr/source/autori:Maglio, Ornella; Costanzo, Salvatore; Cercola, Rosaria; Zambrano, Gerardo; Mauro, Marco; Battaglia, Raffaele; Ferrini, Gianluca; Nastri, Flavia; Pavone, Vincenzo; Lombardi, Angela/titolo:A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction/doi:10.3390%2Fs17122747/rivista:Sensors (Basel)/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume:17 Sensors (Basel, Switzerland) Sensors, Vol 17, Iss 12, p 2747 (2017) Sensors; Volume 17; Issue 12; Pages: 2747 |
| DOI: | 10.3390/s17122747 |
| Popis: | A cost-effective immunosensor for the detection and isolation of dental pulp stem cells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specific monoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCM surface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin–avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with a mixed cell population, was analyzed; the results indicated that the developed QCM immunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell from samples with high complexity, being simple, selective, and providing for more convenient and time-saving operations. |
| Databáze: | OpenAIRE |
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