Nitric Oxide Contributes to the Progression of Myocardial Damage in Experimental Autoimmune Myocarditis in Rats
| Autor: | Masunori Matsuzaki, Takeshi Kasajima, Shinji Abe, Takashi Shimojo, Sakae Ozasa, Hiroe Nakazawa, Michiaki Hiroe, Hiroshi Ito, Fumiaki Marumo, Shigeru Ishiyama, Toshio Nishikawa, Katsutoshi Yamakawa, Minhaz Uddin Mohammed |
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| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Myocarditis In situ hybridization Nitric Oxide Guanidines Autoimmune Diseases Nitric oxide Lesion chemistry.chemical_compound Superoxides Physiology (medical) medicine Animals Cytotoxic T cell Enzyme Inhibitors In Situ Hybridization Autoimmune disease business.industry Myocardium Nitrotyrosine medicine.disease Immunohistochemistry Rats Mononuclear cell infiltration chemistry Rats Inbred Lew Disease Progression Female medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | Circulation. 95:489-496 |
| ISSN: | 1524-4539 0009-7322 |
| Popis: | Background Excess amounts of NO produced by an inducible NO synthase (iNOS) in response to cytokines may be cytotoxic and can be destructive to tissue. We investigated the role of NO in the development of myocardial damage and the effects of aminoguanidine (AG), an inhibitor of iNOS, on experimental autoimmune myocarditis in rats. Methods and Results Autoimmune myocarditis was induced in 20 Lewis rats by injection of porcine cardiac myosin. Ten of the 20 rats were administered AG. The severity of myocarditis was evaluated by measuring the size of myocarditic lesion and serum levels of CK-MB. Serum NO levels were determined using the Cd/Cu method. Tissue specimens were immunohistochemically examined for iNOS and nitrotyrosine. Histopathological study revealed extensive myocardial destruction and massive inflammatory cell infiltration in AG-untreated rats but only focal mononuclear cell infiltration in AG-treated rats. The mean percent areas of inflammatory lesions in the untreated and treated rats were 56±13% and 3±2%, respectively ( P P P Conclusions Excess amounts of NO produced by iNOS appear to contribute to the progression of myocardial damage in myocarditis. AG may prove to be useful in the treatment of myocarditis. |
| Databáze: | OpenAIRE |
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