Evaluation of the drug sensitivity and expression of 16 drug resistance-related genes in canine histiocytic sarcoma cell lines
| Autor: | Hajime Tsujimoto, Yuko Goto-Koshino, Hirotaka Tomiyasu, Koichi Ohno, Hajime Asada, Yasuhito Fujino |
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| Rok vydání: | 2015 |
| Předmět: |
Vincristine
Paclitaxel Cell Drug Evaluation Preclinical canine Antineoplastic Agents Pharmacology Histiocytic sarcoma Biology Real-Time Polymerase Chain Reaction Vinblastine histiocytic sarcoma Dogs Cell Line Tumor Canine Histiocytic Sarcoma Internal Medicine medicine Animals TP53 gene Doxorubicin Dog Diseases Melphalan Etoposide Canine Lymphoma drug resistance Full Paper General Veterinary Reverse Transcriptase Polymerase Chain Reaction Cytarabine medicine.disease Methotrexate Nimustine medicine.anatomical_structure Drug Resistance Neoplasm microtubule inhibitors Cancer research Drug Screening Assays Antitumor medicine.drug |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.14-0415 |
| Popis: | Canine histiocytic sarcoma (HS) is an aggressive tumor type originating from histiocytic cell lineages. This disease is characterized by poor response to chemotherapy and short survival time. Therefore, it is of critical importance to identify and develop effective antitumor drugs against HS. The objectives of this study were to examine the drug sensitivities of 10 antitumor drugs. Using a real-time RT-PCR system, the mRNA expression levels of 16 genes related to drug resistance in 4 canine HS cell lines established from dogs with disseminated HS were determined and compared to 2 canine lymphoma cell lines (B-cell and T-cell). These 4 canine HS cell lines showed sensitivities toward microtubule inhibitors (vincristine, vinblastine and paclitaxel), comparable to those in the canine B-cell lymphoma cell line. Moreover, it was shown that P-gp in the HS cell lines used in this study did not have enough function to efflux its substrate. Sensitivities to melphalan, nimustine, methotrexate, cytarabine, doxorubicin and etoposide were lower in the 4 HS cell lines than in the 2 canine lymphoma cell lines. The data obtained in this study using cultured cell lines could prove helpful in the developing of advanced and effective chemotherapies for treating dogs that are suffering from HS. |
| Databáze: | OpenAIRE |
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