Reorientation of chromosome replication after exposure to ultraviolet light of Escherichia coli
| Autor: | D. Billen, Roger Hewitt |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
DNA
Bacterial Ultraviolet Rays Circular bacterial chromosome In Vitro Techniques Biology Molecular biology Chromosomes Kinetics A-site chemistry.chemical_compound chemistry Control of chromosome duplication Structural Biology Chromosome regions Escherichia coli Ultraviolet light Protein biosynthesis Ultracentrifugation Molecular Biology Mitosis DNA Thymidine |
| Zdroj: | Journal of Molecular Biology. 13:40-53 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/s0022-2836(65)80078-x |
| Popis: | Radioisotopic and density labeling methods have been used to examine the location of the site at which chromosome replication is resumed after the restoration of “net” DNA synthesizing activity in ultraviolet-irradiated cells. The findings were as follows. (1) Post-irradiation chromosome replication commences from a site other than the site that was active prior to exposure. (2) The extent of post-irradiation replication of the total DNA or of a chromosome region which was pulse-labeled immediately prior to exposure is limited to about 50%. (3) All chromosome regions do not have an equal probability of being duplicated following exposure, thus suggesting some specificity in the location of the site where replication is resumed. (4) The observation by others of a requirement for protein synthesis in the restoration of DNA synthesizing activity has been extended to show that inhibition of protein synthesis prevents the appearance of the new replication site. The findings have been interpreted in terms of the currently accepted model for the replication of the bacterial chromosome. |
| Databáze: | OpenAIRE |
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