Quantitative and qualitative changes in CD44 and MEL-14 expression by T cells in C57BL/6 mice during aging
| Autor: | S. Vincent-Naulleau, T. Neway, Henri-Jean Boulouis, F. Barrat, A. Louise, Hélène Haegel, Rhodri Ceredig, Ch. Pilet |
|---|---|
| Přispěvatelé: | Unité mixte de recherche biologie moléculaire et immunologie parasitaires et fongiques, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire - Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) |
| Rok vydání: | 1995 |
| Předmět: |
CD4-Positive T-Lymphocytes
Aging T-Lymphocytes [SDV]Life Sciences [q-bio] Lymphocyte Immunology Spleen CD8-Positive T-Lymphocytes Polymerase Chain Reaction Mice 03 medical and health sciences 0302 clinical medicine Antigen medicine Animals L-Selectin ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Chemistry CD44 T lymphocyte Flow Cytometry Molecular biology Phenotype Mice Inbred C57BL [SDV] Life Sciences [q-bio] Hyaluronan Receptors medicine.anatomical_structure biology.protein Female Lymph CD8 030215 immunology |
| Zdroj: | Research in immunology Research in immunology, Elsevier, 1995, pp.23-34 Research in Immunology Research in Immunology, Elsevier, 1995, pp.23-34 |
| ISSN: | 0923-2494 |
| DOI: | 10.1016/0923-2494(96)80237-9 |
| Popis: | Summary Aging is associated with a decrease in the functional activity of T cells. We have explored age-related alterations in CD44 and MEL-14 expression by spleen cells bearing the Thy1.2, CD4 or CD8 antigens in C57BL/6 mice at 2, 8, 15 and 23 months of age. The membrane expression of CD44 and MEL-14 molecules can be used to distinguish naive (CD44 low , MEL-14 high ) from preactivated/memory (CD44 high , MEL-14 low ) T cells. Our results show that the proportion of CD4 + splenic cells begins to decrease at an intermediate age (8-month-old mice), whereas the proportion of CD8 + cells remains unaltered. The proportion of CD4 + and CD8 + splenic cells with the CD44 high memory phenotype was increased at an early stage of aging (in 8-month-old mice) without a concomitant change in MEL-14 expression. In older mice, MEL-14 expression decreased on CD4 + but not on CD8 + subsets. Recent studies have reported that following activation, the expression of CD44 molecules containing additional, so-called variable exons can be detected. By PCR, we observed an increase in CD44 transcripts containing the v6 or v7 variable exons in murine lymph nodes at the age of 15 months. Our results suggest that v6-or v7-containing variants of CD44 may be involved in the development of memory cells. Taken together, these results suggest that the trafficking of memory T cells in aging may be altered by quantitative and/or qualitative differences in the expression of molecules involved in lymphocyte recirculation. |
| Databáze: | OpenAIRE |
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