Concentrations of p-synephrine in fruits and leaves of Citrus species (Rutaceae) and the acute toxicity testing of Citrus aurantium extract and p-synephrine
| Autor: | Eliane Dallegrave, Marcelo Dutra Arbo, Elisa Rupp Larentis, Renata Pereira Limberger, Ana Lúcia Aboy, Solange Cristina Garcia, Mirna Bainy Leal, A.L. Pimentel, Amélia T. Henriques, Viviane de Moura Linck |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Citrus Orange (colour) Motor Activity Toxicology Body Temperature Mice Oral administration Botany medicine Animals Citrus aurantium extract Acute toxicity testing Chromatography High Pressure Liquid biology Traditional medicine Synephrine Chemistry Reproducibility of Results General Medicine Reference Standards biology.organism_classification Acute toxicity Plant Leaves Rutaceae Fruit Calibration Toxicity Indicators and Reagents Spectrophotometry Ultraviolet Brazil Food Science medicine.drug |
| Zdroj: | Food and Chemical Toxicology. 46:2770-2775 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2008.04.037 |
| Popis: | Dietary supplements containing bitter orange unripe fruit extract/p-synephrine are consumed worldwide for lose weight. This study were conducted to determine the concentration of p-synephrine in unripe fruits and leaves from Citrus aurantium Lin, C. sinensis Osbeck, C. deliciosa Ten, C. limon Burm and C. limonia Osbeck, collected in Southern Brazil, and to evaluate the acute toxicity of C. aurantium extract and p-synephrine. A high performance liquid chromatographic method with diode array detector (HPLC-DAD) was optimized and validated for determination of p-synephrine. The results indicate that all of analyzed samples present p-synephrine in amounts that range from 0.012% to 0.099% in the unripe fruits and 0.029 to 0.438% in the leaves. Acute oral administration of C. aurantium extracts (2.5% p-synephrine, 300-5,000 mg/kg) in mice produced reduction of locomotor activity, p-synephrine (150-2,000 mg/kg) produced piloerection, gasping, salivation, exophtalmia and reduction in locomotor activity, which was confirmed in spontaneous locomotor activity test. All the effects were reversible and persisted for 3-4h. The toxic effects observed seem to be related with adrenergic stimulation and should alert for possible side effects of p-synephrine and C. aurantium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect