In vitro derby imaging of cancer biomarkers using quantum dots
| Autor: | Jung Hwan Lee, Sung Hwan Moon, Hyungu Kang, Won Jun Kang, Do Won Hwang, Dong Soo Lee, Mee Hyang Ko, Hae Young Ko, Soonhag Kim |
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| Rok vydání: | 2009 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Aptamer Fluorescence Biomaterials Imaging Three-Dimensional Cell Line Tumor Quantum Dots Biomarkers Tumor Humans General Materials Science Microscopy Confocal Chemistry technology industry and agriculture RNA-Binding Proteins General Chemistry Aptamers Nucleotide equipment and supplies Integrin alphaVbeta3 Phosphoproteins Molecular biology Small molecule Oligodeoxyribonucleotides Quantum dot Cancer cell Biophysics Cancer biomarkers Nucleolin Oligopeptides Biotechnology |
| Zdroj: | Small (Weinheim an der Bergstrasse, Germany). 5(10) |
| ISSN: | 1613-6829 |
| Popis: | Semiconductor quantum dots (QDs), which have broad absorption with narrow emission spectra, are useful for multiplex imaging. Here, fluorescence derby imaging using dual color QDs conjugated by the AS1411 aptamer (targeting nucleolin) and the arginine-glycine-aspartic acid (targeting the integrin alpha(v)beta(3)) in cancer cells is reported. Simultaneous fluorescence imaging of cellular distribution of nucleolin and integrin alpha(v)beta(3) using QDs enables easy monitoring of separate targets in the cancer cells and the normal healthy cells. These results suggest the feasibility of a concurrent visualization of QD-based multiple cancer biomarkers using small molecules such as aptamer or peptide ligands. |
| Databáze: | OpenAIRE |
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