A Glycosaminoglycan-Rich Fraction from Sea Cucumber Isostichopus badionotus Has Potent Anti-Inflammatory Properties In Vitro and In Vivo
| Autor: | Nuvia Kantún-Moreno, Enrique Hernández-Garibay, Juan José Acevedo, Juan Antonio Pérez-Vega, Alberto Dávalos, Oscar Medina-Contreras, Leticia Olivera-Castillo, Jorge Montero, Cesar Puerto-Castillo, Judit Gil-Zamorano, María A. Fernández-Herrera, Leydi Maribel Carrillo-Cocom, Mayra Pérez-Tapia, Hirian Alonso Moshe Barrera-Perez, Victor May Solís, George Grant, Rossanna Rodríguez-Canul, Miguel A. Olvera-Novoa, Jairo R. Villanueva-Toledo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class Sea Cucumbers Anti-Inflammatory Agents Inflammation lcsh:TX341-641 In Vitro Techniques holothuroids Anti-inflammatory Article Glycosaminoglycan 03 medical and health sciences Mice 0302 clinical medicine In vivo medicine Animals HaCaT Cells Humans Otitis Isostichopus badionotus Mexico Nutrition and Dietetics biology integumentary system ear-inflammation Chemistry Tissue Extracts Chondroitin Sulfates Dextran Sulfate biology.organism_classification Colitis In vitro HaCaT Disease Models Animal 030104 developmental biology Biochemistry glycosaminoglycans inflammation 030220 oncology & carcinogenesis Tetradecanoylphorbol Acetate Tumor necrosis factor alpha medicine.symptom lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Nutrients, Vol 12, Iss 1698, p 1698 (2020) Volume 12 Issue 6 |
| ISSN: | 2072-6643 |
| Popis: | Sea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. Isostichopus badionotus from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that I. badionotus meal had potent anti-inflammatory properties in vivo. We have now screened some of its constituents for anti-inflammatory activity in vitro. Glycosaminoglycan and soluble protein preparations reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in HaCaT cells while an ethanol extract had a limited effect. The primary glycosaminoglycan (fucosylated chondroitin sulfate FCS) was purified and tested for anti-inflammatory activity in vivo. FCS modulated the expression of critical genes, including NF-ĸB, TNF&alpha iNOS, and COX-2, and attenuated inflammation and tissue damage caused by TPA in a mouse ear inflammation model. It also mitigated colonic colitis caused in mice by dextran sodium sulfate. FCS from I. badionotus of the Yucatan Peninsula thus had strong anti-inflammatory properties in vivo. |
| Databáze: | OpenAIRE |
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