Blueberry extract decreases oxidative stress and improves functional parameters in lungs from rats with pulmonary arterial hypertension
| Autor: | Fabiana Agostini, Denise dos Santos Lacerda, Schauana Freitas Fraga, Isadora Schein Salvador, Cristina Campos-Carraro, Mariana Koetz, Alex Sander da Rosa Araujo, Alan Christhian Bahr, Amélia T. Henriques, Patrick Turck, Vanessa Duarte Ortiz, Alexandre Roberto Hickmann, Adriane Belló-Klein |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Endocrinology Diabetes and Metabolism Blueberry Plants 030209 endocrinology & metabolism Pulmonary Artery Pharmacology medicine.disease_cause Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lipid oxidation medicine.artery Animals Medicine Arterial Pressure Rats Wistar Xanthine oxidase Lung Pulmonary Arterial Hypertension Monocrotaline 030109 nutrition & dietetics Nutrition and Dietetics Plant Extracts business.industry medicine.disease Pulmonary hypertension Rats Disease Models Animal medicine.anatomical_structure chemistry Pulmonary artery business Endothelin receptor Oxidation-Reduction Nicotinamide adenine dinucleotide phosphate Oxidative stress |
| Zdroj: | Nutrition. 70:110579 |
| ISSN: | 0899-9007 |
| DOI: | 10.1016/j.nut.2019.110579 |
| Popis: | Objectives Pulmonary arterial hypertension (PAH) is a condition characterized by an increased resistance of pulmonary vasculature, culminating in an increase in pulmonary pressure. This process involves disturbances in lung redox homeostasis, causing progressive right heart failure. In this context, the use of natural antioxidants, such as those found in blueberries, may represent a therapeutic approach. The aim of this study was to evaluate the effect of blueberry extract (BB) on functional parameters and oxidative stress levels in rat lungs with induced PAH. Methods Forty-eight male Wistar rats (weighing 200 ± 20 g) were randomized into five groups: control, monocrotaline, monocrotaline + BB 50, monocrotaline + BB 100, and monocrotaline + BB 200. PAH was induced by the administration of monocrotaline (60 mg/kg, intraperitoneal). Rats were treated with BB at doses of 50, 100, and 200 mg/kg via gavage for 5 wk (2 wk before monocrotaline and 3 wk after monocrotaline injection). At day 35, rats were submitted to echocardiography and catheterization. They were then sacrificed and lungs were harvested for biochemical analyses. Results BB increased the E/A ratio of blood flow across the tricuspid valve and tricuspid annular phase systolic excursion, as wells as decreased the mean pulmonary artery pressure of animals compared with the PAH group. Moreover, BB decreased total reactive species concentration and lipid oxidation, reduced activity of nicotinamide adenine dinucleotide phosphate oxidase and expression of xanthine oxidase, increased the activity of superoxide dismutase and restored sulfhydryl content in the animal lungs compared with those in the PAH group. Additionally, BB restored expression of the antioxidant transcriptional factor Nrf2 in the lungs of the animal subjects. Finally, BB normalized the endothelin receptor (ETA/ETB) expression ratio in the animal lungs, which were increased in the PAH group. Conclusion Intervention with BB mitigated functional PAH outcomes through improvement of the pulmonary redox state. Our results provide a basis for future research on natural antioxidant interventions as a novel treatment strategy in PAH. |
| Databáze: | OpenAIRE |
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