Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display
| Autor: | Gitali Ganguli, Christine Wasylyk, Inès Schultz, A. Cromer, Annaïck Carles, Régine Millon, Olivier Poch, F Lemaire, Y. Rabouel, C. Ducray, L. Bracco, C. Zhao, Danièle Muller, Julia Young, P. Marchal, Doulaye Dembélé, Joseph Abecassis, Bohdan Wasylyk |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
MESH: Sequence Analysis DNA Proteome Gene Expression MESH: Amino Acid Sequence MESH: Base Sequence Genome Transcriptome 0302 clinical medicine MESH: Reverse Transcriptase Polymerase Chain Reaction Oligonucleotide Array Sequence Analysis Genetics 0303 health sciences Expressed sequence tag Differential display Reverse Transcriptase Polymerase Chain Reaction MESH: Protein Array Analysis MESH: Genomics MESH: Carcinoma Squamous Cell DNA Neoplasm Genomics Cell cycle Immunohistochemistry MESH: Proteome Head and Neck Neoplasms 030220 oncology & carcinogenesis Carcinoma Squamous Cell MESH: Computational Biology MESH: Gene Expression Molecular Sequence Data Protein Array Analysis MESH: DNA Neoplasm Computational biology Biology 03 medical and health sciences MESH: Gene Expression Profiling medicine Humans MESH: Blotting Northern Amino Acid Sequence Molecular Biology 030304 developmental biology MESH: Humans MESH: Molecular Sequence Data Base Sequence Cell growth Gene Expression Profiling Computational Biology MESH: Immunohistochemistry [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Sequence Analysis DNA medicine.disease Blotting Northern Head and neck squamous-cell carcinoma MESH: Head and Neck Neoplasms MESH: Oligonucleotide Array Sequence Analysis |
| Zdroj: | Oncogene Oncogene, Nature Publishing Group, 2006, 25 (12), pp.1821-31. ⟨10.1038/sj.onc.1209203⟩ |
| ISSN: | 0950-9232 1476-5594 |
| DOI: | 10.1038/sj.onc.1209203⟩ |
| Popis: | International audience; Head and neck squamous cell carcinoma (HNSCC) is common worldwide and is associated with a poor rate of survival. Identification of new markers and therapeutic targets, and understanding the complex transformation process, will require a comprehensive description of genome expression, that can only be achieved by combining different methodologies. We report here the HNSCC transcriptome that was determined by exhaustive differential display (DD) analysis coupled with validation by different methods on the same patient samples. The resulting 820 nonredundant sequences were analysed by high throughput bioinformatics analysis. Human proteins were identified for 73% (596) of the DD sequences. A large proportion (>50%) of the remaining unassigned sequences match ESTs (expressed sequence tags) from human tumours. For the functionally annotated proteins, there is significant enrichment for relevant biological processes, including cell motility, protein biosynthesis, stress and immune responses, cell death, cell cycle, cell proliferation and/or maintenance and transport. Three of the novel proteins (TMEM16A, PHLDB2 and ARHGAP21) were analysed further to show that they have the potential to be developed as therapeutic targets. |
| Databáze: | OpenAIRE |
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