Cerebral excitability in pup rats prenatally exposed to 1-bromopropane is suppressed by bromide accumulated in the brain
| Autor: | Mitsuo Hinoue, Toru Ishidao, C Edwin Garner, Hajime Hori, Mizuki Matsuno, Yukiko Fueta, Hazuki Noguchi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Bromides
medicine.medical_specialty Health Toxicology and Mutagenesis Hippocampal formation 1-bromopropane chemistry.chemical_compound Bromide Pregnancy Internal medicine Prenatal exposure medicine Neurotoxicity Animals Rats Wistar Inhalation Chemistry Hippocampal slice Public Health Environmental and Occupational Health Brain medicine.disease 1-Bromopropane Hydrocarbons Brominated Rats Endocrinology Prenatal Exposure Delayed Effects Gestation Pentylenetetrazole Female Original Article Artificial cerebrospinal fluid |
| Zdroj: | Industrial Health |
| ISSN: | 1880-8026 |
| Popis: | Previously, we reported that prenatal exposure to 1-bromopropane (1-BP) causes the accumulation of bromide (Br-) in the brain of rat pups. Here, we aimed to investigate the effects of Br- accumulation in rat pups prenatally exposed to 1-BP vapor. Dam rats were exposed to 1-BP (400 or 700 ppm; 1-BP group) by inhalation, or to NaBr (20 mM; Br- group) in drinking water during gestation days 1-20. We also analyzed pentylenetetrazole (PTZ, 60 mg/kg, ip)-induced behavioral changes in pups prenatally exposed to 1-BP or Br- on postnatal day (PND) 14. PTZ-induced epileptic convulsions were inhibited in both 1-BP (700 ppm) and Br- groups. The inhibition of neuronal excitability induced by Br- was evaluated electrophysiologically using the hippocampal slices obtained from PND14-16 pups. PTZ (2 mM) failed to induce epileptiform discharge in the presence of 1.2 mM Br- in the slices obtained from the control group. However, it induced epileptiform discharge following the removal of Br-, by perfusing artificial cerebrospinal fluid into the slices obtained from the Br- group. Our results indicate that Br- accumulates in the brain of neonatal rat pups prenatally exposed to 1-BP vapor suppressed neuronal excitability. |
| Databáze: | OpenAIRE |
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