Evaluation of Antibacterial Activities of Citrus limon, Citrus reticulata, and Citrus grandis Against Pathogenic Bacteria
Autor: | Mansour Amin, Reza Seyfi Gooybari, Sholeh Saeb, Nasrin Aghel |
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Rok vydání: | 2016 |
Předmět: |
medicine.drug_class
Extract Microorganism Antibiotics Citrus limon 02 engineering and technology medicine.disease_cause 01 natural sciences Essential oil law.invention law Botany medicine E test Food science Citrus grandis pathogen bacteria lcsh:RC799-869 biology 010405 organic chemistry food and beverages Pathogenic bacteria 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification 0104 chemical sciences Staphylococcus aureus lcsh:Diseases of the digestive system. Gastroenterology Antibacterial activity Citrus reticulata 0210 nano-technology Bacteria |
Zdroj: | International Journal of Enteric Pathogens, Vol 4, Iss 4, p e37103 (2016) |
ISSN: | 2322-5866 2345-3362 |
DOI: | 10.15171/ijep.2016.13 |
Popis: | Background: Microorganisms resistant to most antibiotics are rapidly spreading, and there is an urgent and continuous need for novel antimicrobial compounds. The genus Citrus belongs to the family Rutaceae has many biologically active secondary metabolites. Objectives: The purpose of this study was to evaluate antimicrobial activity of essential oil and extract of Lemon (Citrus limon), Mandarin (Citrus reticulata) and Pummelo (Citrus grandis) against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella typhi. Materials and Methods: The fresh Citrus leaves were shade-dried and powdered. Antimicrobial metabolites were extracted from them by 80% methanol for extract and using a Clevenger-type apparatus for essential oil. Eight different concentrations of the each leaf extract and essential oil were prepared. The antimicrobial susceptibility assay of Citrus leaves metabolites were subjected against four bacterial strains by agar disc diffusion and E-test method. Results: In this study, minimum inhibitory concentrations (MIC) of different Citrus leaf extracts were determined against all four food-borne pathogens. The C. grandis leaf essential oil had potent antimicrobial activity against all four pathogens, and the C. limon leaf essential oil was effective on Gram-positive bacteria. S. typhi was resistant against two leaves essential oils. Conclusions: The results showed that there was no antimicrobial activity effect in all extracts on tested bacteria. In this study, the antibacterial effect of essential oil of Citrus leaves on four strains of pathogenic microorganisms was confirmed. The C. grandis leaf essential oil had the most powerful antimicrobial properties, suggesting its potential application as natural preservative in foods or an effective medicine against different pathogenic microbes. Key words: Antibacterial activity, E-test, Citr |
Databáze: | OpenAIRE |
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