Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development
| Autor: | Hazel Y. Stevens, Sanjay Sridaran, Tanushree Thote, S. Moran, Yazdan Raji, Angela S.P. Lin, Nick J. Willett, Robert E. Guldberg |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.medical_treatment Immunology Anti-Inflammatory Agents Arthritis Osteoarthritis Injections Intra-Articular Rheumatology medicine Immunology and Allergy Synovial fluid Animals Humans Amnion Saline business.industry Cartilage Dehydrated Human Amnion/Chorion Membrane Chorion medicine.disease Arthritis Experimental Rats medicine.anatomical_structure Rats Inbred Lew Synovial membrane business Research Article |
| Zdroj: | Arthritis Research & Therapy |
| ISSN: | 1478-6362 |
| Popis: | Introduction Micronized dehydrated human amnion/chorion membrane (μ-dHACM) is derived from donated human placentae and has anti-inflammatory, low immunogenic and anti-fibrotic properties. The objective of this study was to quantitatively assess the efficacy of μ-dHACM as a disease modifying intervention in a rat model of osteoarthritis (OA). It was hypothesized that intra-articular injection of μ-dHACM would attenuate OA progression. Methods Lewis rats underwent medial meniscal transection (MMT) surgery to induce OA. Twenty four hours post-surgery, μ-dHACM or saline was injected intra-articularly into the rat joint. Naïve rats also received μ-dHACM injections. Microstructural changes in the tibial articular cartilage were assessed using equilibrium partitioning of an ionic contrast agent (EPIC-μCT) at 21 days post-surgery. The joint was also evaluated histologically and synovial fluid was analyzed for inflammatory markers at 3 and 21 days post-surgery. Results There was no measured baseline effect of μ-dHACM on cartilage in naïve animals. Histological staining of treated joints showed presence of μ-dHACM in the synovium along with local hypercellularity at 3 and 21 days post-surgery. In MMT animals, development of cartilage lesions at 21 days was prevented and number of partial erosions was significantly reduced by treatment with μ-dHACM. EPIC-μCT analysis quantitatively showed that μ-dHACM reduced proteoglycan loss in MMT animals. Conclusions μ-dHACM is rapidly sequestered in the synovial membrane following intra-articular injection and attenuates cartilage degradation in a rat OA model. These data suggest that intra-articular delivery of μ-dHACM may have a therapeutic effect on OA development. |
| Databáze: | OpenAIRE |
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