In vitro Studies and Clinical Observations Imply a Synergistic Effect Between Epstein-Barr Virus and Dengue Virus Infection
| Autor: | Lingzhai Zhao, Xue-Ying Liang, Xiao-Mei Deng, Lei Yu, Zhong-Yu Liu, Pei Xu, Dan Li, Hua Zhang, Fuchun Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) reactivation viruses 030106 microbiology Dengue virus Biology medicine.disease_cause Peripheral blood mononuclear cell Microbiology Virus Dengue fever Epstein–Barr virus 03 medical and health sciences hemic and lymphatic diseases medicine dengue fever Original Research dengue virus virus diseases biochemical phenomena metabolism and nutrition medicine.disease Virology coinfection QR1-502 030104 developmental biology Cell culture Superinfection Coinfection |
| Zdroj: | Frontiers in Microbiology, Vol 12 (2021) Frontiers in Microbiology |
| DOI: | 10.3389/fmicb.2021.691008/full |
| Popis: | Dengue virus (DENV) infection can lead to a complex spectrum of clinical outcomes, ranging from asymptomatic infection to life-threatening severe dengue. The reasons for thus drastically varying manifestations of the disease remain an enigma. Herein, we reported an original discovery of the synergistic effect between preexisting Epstein–Barr virus (EBV) infection and DENV superinfection in vitro and of a strong correlation of these two viruses in the clinical samples from dengue patients. We showed that (I) DENV-2 infection of an EBV-positive cell line (EBV + Akata cell) reactivated EBV, and it could be blocked by wortmannin treatment. (II) Examination of human peripheral blood mononuclear cell (PBMC) samples from dengue patients revealed significantly elevated cell-associated EBV DNA copy number at the time of hospitalization vs. at the time of disease recovery in most individuals. (III) EBV infection promoted DENV propagation in both EBV-hosting B cells and indirectly in THP-1 cells, supported by the following evidence: (A) EBV + Akata cells were more permissive to DENV-2 infection compared with Akata cells harboring no EBV virus (EBV- Akata cells). (B) Low-molecular weight fraction secreted from EBV + Akata cells could enhance DENV-2 propagation in monocytic THP-1 cells. (C) While reactivation of EBV in EBV + Akata cells further increased DENV-2 yield from this cell line, pharmacological inhibition of EBV replication by acyclovir had the opposite effect. To our knowledge, this is the first investigation demonstrating a positive correlation between EBV and DENV in vitro and in human biospecimens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|