Increasing Accuracy and Throughput in Large-Scale Microsatellite Fingerprinting of Cacao Field Germplasm Collections
| Autor: | Pathmanathan Umaharan, Dapeng Zhang, Michel Boccara, Stephen Pinney, Lambert A. Motilal, Sue Mischke |
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| Rok vydání: | 2008 |
| Předmět: |
Germplasm
Identification Séquence nucléotidique Locus Theobroma ADN Pooling Locus (genetics) Plant Science Biology F30 - Génétique et amélioration des plantes Genetics Theobroma cacao Marqueur génétique Dna pooling Microsatellite biology.organism_classification Evolutionary biology Collection de matériel génétique Identity resolution Empreinte ADN |
| Zdroj: | Tropical Plant Biology |
| ISSN: | 1935-9764 1935-9756 |
| DOI: | 10.1007/s12042-008-9016-z |
| Popis: | In this study we report on increasing the rate and accuracy of microsatellite fingerprinting of accessions in Theobroma cacao L. field germplasm collections with a medium-throughput capillary sequencing system. We examined the use of a reduced number of microsatellite loci to decrease the time and materials required for fingerprinting and determined the best available microsatellite loci for accurately separating accessions. A subset of nine informative loci that could separate sixty accessions into the same genetic groupings as a complete set of 37 loci was found. Stringent probability of identity values (i.e. chance of unique accession) was highly influenced (r = −0.996; P |
| Databáze: | OpenAIRE |
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