Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast
| Autor: | Pernilla Bjerling, Karl Ekwall, Yeun Kyu Jang, Rho Hyun Seong, Sang Dai Park, Myung Ae Lee, Eung Jae Yoo, Jae Bum Kim |
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| Rok vydání: | 2002 |
| Předmět: |
Transcription
Genetic Cell Survival Blotting Western Molecular Sequence Data Biophysics Chromatin Remodeling Factor Biology Biochemistry Chromatin remodeling Chromodomain Histones Non-histone protein Heterochromatin Schizosaccharomyces Amino Acid Sequence Gene Silencing Molecular Biology Phylogeny Adenosine Triphosphatases Cell Nucleus Sequence Homology Amino Acid DNA Cell Biology ChIP-on-chip Precipitin Tests Molecular biology Chromatin Protein Structure Tertiary ChIP-sequencing Cell biology DNA-Binding Proteins Phenotype Microscopy Fluorescence Mutation Heterochromatin protein 1 Gene Deletion Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 295:970-974 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(02)00797-0 |
| Popis: | Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3(+) is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo. |
| Databáze: | OpenAIRE |
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