Organisation and sequence analysis of nuclear-encoded 5s ribosomal RNA genes in cryptomonad algae
| Autor: | Gregory J. Adcock, Barbara J. Howlett, Geoffrey I. McFadden, Paul R. Gilson |
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| Rok vydání: | 1995 |
| Předmět: |
Genetics
Polymorphism Genetic Nuclear gene Base Sequence Eukaryotic Large Ribosomal Subunit Molecular Sequence Data 5.8S ribosomal RNA RNA Ribosomal 5S Eukaryota General Medicine Biology Ribosomal RNA DNA Ribosomal Polymerase Chain Reaction Molecular biology 18S ribosomal RNA 5S ribosomal RNA 23S ribosomal RNA Sequence Homology Nucleic Acid Internal transcribed spacer |
| Zdroj: | Current Genetics. 27:239-242 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00326155 |
| Popis: | Southern hybridisation, polymerase chain reaction (PCR), and nucleotide sequence, data indicate that the 5s ribosomal RNA (rRNA) gene is linked to the main rRNA gene repeat in the nuclear genome of four cryptomonad algae (Rhinomonas pauca, Storeatula major, Komma caudata, and isolate Cs 134). The 5s gene is apparently transcribed in the same direction as the large and small subunit rRNA genes. The intergenic spacer between the 5s gene and the large subunit rRNA gene exhibits length and sequence polymorphism among the different species. Cryptomonads contain two different eukaryotic genomes: the host nucleus and the nucleus of a eukaryotic endosymbiont. Mapping experiments with isolated chromosomes of the host and endosymbiont genomes showed that the intergenic spacer between the large subunit and the 5s rRNA gene, which was amplified from total DNA by PCR, was derived from the host nuclear genome. |
| Databáze: | OpenAIRE |
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