Neurotrophin-7: a novel member of the neurotrophin family from the zebrafish
| Autor: | Carlos F. Ibáñez, Mike Fainzilber, Ann-Sofie Nilsson, Pierre Falck |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
p75
Evolution Receptor tyrosine kinase Molecular Sequence Data Biophysics Neurotrophic factor Receptors Nerve Growth Factor Tropomyosin receptor kinase B Tropomyosin receptor kinase A Polymerase Chain Reaction Receptor Nerve Growth Factor Biochemistry Tropomyosin receptor kinase C Brain-derived neurotrophic factor chemistry.chemical_compound Nerve growth factor Structural Biology Genetics Animals Humans Low-affinity nerve growth factor receptor Amino Acid Sequence Nerve Growth Factors Molecular Biology Phylogeny Zebrafish Base Sequence Sequence Homology Amino Acid biology Tyrosine phosphorylation Cell Biology Zebrafish Proteins Recombinant Proteins Rats Cell biology nervous system chemistry Culture Media Conditioned COS Cells biology.protein Neurotrophin |
| Zdroj: | FEBS Letters. 424:285-290 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(98)00192-6 |
| Popis: | A novel member of the neurotrophin family, zebrafish neurotrophin-7 (zNT-7), was isolated from the zebrafish Danio rerio. The amino acid sequence of zNT-7 is more closely related to that of fish nerve growth factor (NGF) and neurotrophin-6 (NT-6) than to that of any other neurotrophin. zNT-7 is, however, equally related to fish NGF and NT-6 (65% and 63% amino acid sequence identity, respectively) indicating that it represents a distinct neurotrophin sequence. zNT-7 contains a 15 amino acid residue insertion in a β-turn region in the middle of the mature protein. Recombinant zNT-7 was able to bind to the human p75 neurotrophin receptor and to induce tyrosine phosphorylation of the rat TrkA receptor tyrosine kinase, albeit less efficiently than rat NGF. zNT-7 did not interact with rat TrkB or TrkC, indicating a similar receptor specificity as NGF. We propose that a diversification of the NGF subfamily in the neurotrophin evolutionary tree occurred during the evolution of teleost fishes which resulted in the appearance of several additional members, such as zNT-7 and NT-6, structurally and functionally related to NGF. |
| Databáze: | OpenAIRE |
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