Diagnosing human cutaneous leishmaniasis using fluorescence in situ hybridization
| Autor: | Subodha Jayathilake, Shalindra Ranasinghe, Chandranie Menike, Renu Wickremasinghe, Thilini Dilhara Jayasena Kaluarachchi, S.G. Yasawardene, Rajitha Wickremasinghe, Bandujith Yapa, Anuradha Munasinghe, Manjula Weerasekera, Andrew J. McBain, Hiromel De Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.diagnostic_test business.industry health care facilities manpower and services Public Health Environmental and Occupational Health Histology social sciences General Medicine medicine.disease Microbiology Infectious Diseases Cutaneous leishmaniasis parasitic diseases medicine Parasitology Sri lanka business health care economics and organizations geographic locations Fluorescence in situ hybridization |
| Zdroj: | Jayasena Kaluarachchi, T, Wickremasinghe, R, Weerasekera, M, Yasawardene, S, Mcbain, A J, Yapa, B, De Silva, H, Menike, C, Jayathilake, S, Munasinghe, A, Wickremasinghe, R & Ranasinghe, S 2021, ' Diagnosing human cutaneous leishmaniasis using fluorescence in situ hybridization ', Pathogens and Global Health, pp. 1-8 . https://doi.org/10.1080/20477724.2021.1896265 |
| Popis: | Cutaneous leishmaniasis (CL) is endemic in Sri Lanka. Giemsa-stained slit-skin-smears (SSS-Giemsa) and histology are routinely used in diagnosis with a sensitivity of 40–70%. PCR currently has limited accessibility. Therefore, we assessed the sensitivity and specificity of a previously described fluorescence in situ hybridization assay, on skin smears and biopsy samples to overcome the limitations encountered with routine diagnostic methods. Samples from a total of 123 suspected CL patients were collected and subjected to SSS-Giemsa, fluorescence in situ hybridization (FISH) on slit skin smears (SSS-FISH), formalin-fixed-paraffin-embedded-tissues stained with Hematoxylin & Eosin staining (FFPE-H&E) and FISH on formalin-fixed-paraffin-embedded-tissues (FFPE-FISH). Negative controls of 61 patient samples were collected from a CL non-endemic area and subjected to the same procedures. The gold standard PCR was used as a comparator. For FISH, two previously described cyanine 3 tagged Leihsmania genus-specific probes were used. Compared to PCR, SSS-Giemsa, SSS-FISH, FFPE-H&E, and FFPE-FISH had sensitivities of 76.5%, 79.1%, 50.4% and 80.9%, respectively. Routine diagnostic tests (SSS-Giemsa and FFPE-H&E) had a specificity of 100%. SSS-FISH and FFPE-FISH had specificities of 96.7% and 93.4%, respectively. FFPE-FISH had a statistically significant higher diagnostic performance than FFPE-H&E (p < 0.001). The relative performance of SSS-Giemsa, SSS-FISH and FFPE-FISH was similar (p > 0.05 for all comparisons). We conclude that FFPE-FISH is a more accurate diagnostic tool than FFPE–H&E. SSS-FISH did not have an additional advantage over SSS-Giemsa in diagnosis. However, SSS-FISH could be recommended as a minimally invasive method in studies assessing wound healing where immunological probes are used. |
| Databáze: | OpenAIRE |
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