Characterization ofgtf, a Glucosyltransferase Gene in the Genomes ofPediococcus parvulusandOenococcus oeni, Two Bacterial Species Commonly Found in Wine
| Autor: | Claire Le Marrec, Gérard Chambat, Aline Lonvaud-Funel, Alain Heyraud, Marguerite Dols-Lafargue, Hyo Young Lee |
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| Přispěvatelé: | Oenologie (UMRO), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2008 |
| Předmět: |
GÈNE GTF
beta-Glucans Molecular Sequence Data Mutant Wine Polymerase Chain Reaction Applied Microbiology and Biotechnology Bacterial Adhesion Microbiology Industrial Microbiology 03 medical and health sciences PEDIOCOCCUS PARVULUS stomatognathic system Pediococcus Gene 030304 developmental biology Oenococcus oeni [SDV.EE]Life Sciences [q-bio]/Ecology environment 0303 health sciences Ecology biology OENOCOCCUS OENI Reverse Transcriptase Polymerase Chain Reaction 030306 microbiology Sequence Analysis DNA biology.organism_classification Recombinant Proteins Gram-Positive Cocci stomatognathic diseases Biochemistry Glucosyltransferases Mutation Food Microbiology biology.protein Glucosyltransferase Mobile genetic elements Heat-Shock Response Bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (13), pp.4079-4090. ⟨10.1128/AEM.00673-08⟩ |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | “Ropiness” is a bacterial alteration in wines, beers, and ciders, caused by β-glucan-synthesizing pediococci. A single glucosyltransferase, Gtf, controls ropy polysaccharide synthesis. In this study, we show that the correspondinggtfgene is also present on the chromosomes of several strains ofOenococcus oeniisolated from nonropy wines.gtfis surrounded by mobile elements that may be implicated in its integration into the chromosome ofO. oeni. gtfis expressed in all thegtf+strains, and β-glucan is detected in the majority of these strains. Part of this β-glucan accumulates around the cells forming a capsule, while the other part is liberated into the medium together with heteropolysaccharides. Most of the time, this polymer excretion does not lead to ropiness in a model medium. In addition, we show that wild or recombinant bacterial strains harboring a functionalgtfgene (gtf+) are more resistant to several stresses occurring in wine (alcohol, pH, and SO2) and exhibit increased adhesion capacities compared to theirgtfmutant variants. |
| Databáze: | OpenAIRE |
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