Microfluidic device for rapid digestion of tissues into cellular suspensions
Autor: | Elliot E. Hui, Jered B. Haun, Erik M. Werner, Pedram P. Pourfard, Amrith A. Karunaratne, Edward L. Nelson, Xiaolong Qiu, Trisha M. Westerhof |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Materials science Cell Survival Cells Microfluidics Sample processing Biomedical Engineering Bioengineering 02 engineering and technology Regenerative Medicine Kidney Biochemistry Regenerative medicine Article Analytical Chemistry 03 medical and health sciences Mice Engineering Digestion (alchemy) Tissue engineering Lab-On-A-Chip Devices Animals Viability assay Cells Cultured Cultured Tissue Engineering Proteolytic enzymes General Chemistry Equipment Design Microfluidic Analytical Techniques Stem Cell Research 021001 nanoscience & nanotechnology 030104 developmental biology Liver Chemical Sciences Stem cell 0210 nano-technology Biotechnology Biomedical engineering |
Zdroj: | Lab on a chip, vol 17, iss 19 |
ISSN: | 1473-0189 |
Popis: | The ability to harvest single cells from tissues is currently a bottleneck for cell-based diagnostic technologies, and remains crucial in the fields of tissue engineering and regenerative medicine. Tissues are typically broken down using proteolytic digestion and various mechanical treatments, but success has been limited due to long processing times, low yield, and high manual labor burden. Here, we present a novel microfluidic device that utilizes precision fluid flows to improve the speed and efficiency of tissue digestion. The microfluidic channels were designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. We show using animal organs that our digestion device with hydro-mincing capabilities was superior to conventional scalpel mincing and digestion based on recovery of DNA and viable single cells. Thus, our microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. We envision our novel device being used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine. |
Databáze: | OpenAIRE |
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