In vitro experiment to elucidate the mechanism of the 'soft shell technique' for preventing subretinal migration of perfluoro-octane
| Autor: | Taiji Sakamoto, Yau Kei Chan, HC Cheng, Jing Wu, David Wong, Rumana Hussain, Gabriela Czanner, Ho Cheung Shum, Yongjie Lu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Perfluoro-octane genetic structures Swine Shell (structure) Vitreoretinal Surgery 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Postoperative Complications medicine Animals Hyaluronic Acid Retina Fluorocarbons Ha coating Viscosupplements business.industry Drop (liquid) Vitreoretinal surgery In vitro experiment Retinal Perforations Sensory Systems Surgery Ophthalmology Disease Models Animal 030104 developmental biology medicine.anatomical_structure 030221 ophthalmology & optometry business Biomedical engineering Retinal hole |
| Zdroj: | The British journal of ophthalmology. 101(3) |
| ISSN: | 1468-2079 |
| Popis: | Perfluorocarbon liquid (PFCL) can migrate into subretinal space in detached and stiffened retina with open holes during vitreoretinal surgery. An innovative 'soft shell' technique was introduced to reduce the complication using hyaluronate (HA) to 'cover' the retinal hole. This study aims to study the effectiveness of this technique in vitro.Ex vivo porcine retina was mounted on a transwell insert. Beneath the retina was an aqueous solution. Two retinal holes were made using needle punctures. One of the two retinal holes was covered with HA. Perfluoro-n-octane (PFO) was added above the retina incrementally using a syringe pump. The height of PFO required to cause the migration of PFO through the retinal holes was measured. The 'pendant drop' method was carried out to measure the interfacial tensions between the PFO and aqueous, and between PFO and four different concentrations of HA solution.A statistically higher PFO level was required to cause the migration of PFO through the retinal hole with HA coating than without HA coating (Tobit regression with p0.05). The use of HA was associated with 2.39-fold increase in hydrostatic pressure before the collapse of the PFO interface at the retinal holes. The interfacial tension between PFO and HA solution with concentrations of 0.05%, 0.25%, 0.5% and 1% were 54.2±0.6, 55.3±0.6, 59.5±1.5 and 68.3±1.3 mN/m, respectively (mean±SD). The interfacial tension between PFO and aqueous with 1% HA coating (68.3±1.3 mN/m) was significantly higher than that without (37.4±3.4 mN/m) (p0.05).The interfacial tension between HA and PFO is higher than that between aqueous and PFO. This is a plausible physical explanation of how the 'soft shell' technique might work to prevent subretinal migration of PFCL. |
| Databáze: | OpenAIRE |
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