Rapid and sensitive detection of Mycobacterium tuberculosis based on strand displacement amplification and magnetic beads
| Autor: | Sai Jin Xiao, Guang Mei Zhang, Zhibin Zhang, Zhao Jun Chu, Ming Yue Yuan, Shan Ping Wang, Li Zhi Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Tuberculosis
Biophysics 02 engineering and technology 01 natural sciences Polymerase Chain Reaction Sensitivity and Specificity law.invention Mycobacterium tuberculosis chemistry.chemical_compound Tuberculosis diagnosis law medicine Humans Pathogen Polymerase chain reaction biology Magnetic Phenomena 010401 analytical chemistry Multiple displacement amplification 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease Virology 0104 chemical sciences chemistry Chemistry (miscellaneous) 0210 nano-technology Bacteria DNA |
| Zdroj: | Luminescence : the journal of biological and chemical luminescenceREFERENCES. 36(1) |
| ISSN: | 1522-7243 |
| Popis: | Tuberculosis is one of the main infectious diseases threatening public health, and the development of simple, rapid, and cost-saving methods for tuberculosis diagnosis is of profound importance for tuberculosis prevention and treatment. The bacterium Mycobacterium tuberculosis (MTB) is the pathogen that causes tuberculosis, and assaying for MTB is the only criterion for tuberculosis diagnosis. A new enzyme-free method based on strand displacement amplification and magnetic beads was developed for simple, rapid, and cost-saving MTB detection. Under optimum conditions, a good linear relationship could be observed between fluorescence and MTB specific DNA concentration ranging from 0.05 to 150 nM with a correlation coefficient of 0.993 (n = 8) and a detection limit of 47 pM (3σ/K). The present method also distinguished a one base mismatch from MTB specific DNA, showing great promise for MTB genome single base polymorphism analysis. MTB specific DNA content in polymerase chain reaction samples was successfully detected using the new method, and recoveries were 97.8-100.8%, indicating that the present method had high accuracy and shows good potential for the early diagnosis of tuberculosis. |
| Databáze: | OpenAIRE |
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