Localization of factor VIII-procoagulant antigen: an immunohistological survey of the human body using monoclonal antibodies
| Autor: | E. Cristen, T.H. Van Der Kwast, EC Veerman, HV Stel, RM Bertina |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class Kupffer Cells Immunology Spleen Monoclonal antibody Biochemistry Antigen Bone Marrow von Willebrand Factor medicine Humans Endothelium Antigens Cells Cultured Factor VIII biology Macrophages Antibodies Monoclonal Mononuclear phagocyte system Cell Biology Hematology medicine.anatomical_structure Liver biology.protein Red pulp Blood Vessels Bone marrow Lymph Antibody |
| Zdroj: | Blood. 67(1) |
| ISSN: | 0006-4971 |
| Popis: | Various organs, including liver, spleen, heart, lung, kidney, intestines, lymph nodes, pancreas, bone marrow, and thymus, were investigated for the presence of factor VIII-procoagulant antigen (VIIICAg) and factor VIII-related antigen (VIIIRAg), using a panel of monoclonal antibodies directed to factor VIII-von Willebrand factor in combination with a sensitive immunoperoxidase staining technique. In addition to hepatic sinusoidal endothelial cells, the presence of VIIICAg was demonstrated in mononuclear cells sporadically present in lymph nodes, in the alveolar septa of lung, and in the red pulp of spleen. The identity of these mononuclear cells could not be unequivocally determined. Based on morphological criteria, however, it is tentatively concluded that these cells are nonlymphoid and belong to the mononuclear phagocyte system. The presence of VIII-RAg was confined to vascular endothelial cells, hepatic sinusoidal endothelial cells, cells lining the venous sinuses of the red pulp of the spleen, cells lining renal glomeruli and lung capillaries, platelets, and megakaryocytes. |
| Databáze: | OpenAIRE |
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