PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain
| Autor: | Dominic Lambert, George Huszczynski, Martine Gauthier, Michael Knowles, Burton W. Blais |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Strain (chemistry) Sequence analysis Computational Biology Pathogenic bacteria Genomic signature Food Contamination Sequence Analysis DNA Biology medicine.disease_cause Escherichia coli O157 Microbiology Polymerase Chain Reaction Bacterial genetics law.invention law medicine Food Microbiology Food microbiology Escherichia coli Polymerase chain reaction Genome Bacterial Food Science DNA Primers |
| Zdroj: | Journal of food protection. 78(9) |
| ISSN: | 1944-9097 |
| Popis: | Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in the analytical procedure. Before positive findings can be reported for risk management purposes, analysts must have a means of verifying that pathogenic bacteria (e.g., E. coli O157:H7) recovered from test samples are not due to inadvertent contamination with the control strain routinely handled in the laboratory environment. Here, we report on the application of an in-house bioinformatic pipeline for the identification of unique genomic signature sequences in the development of specific oligonucleotide primers enabling the identification of a common positive control strain, E. coli O157:H7 (ATCC 35150), using a simple PCR procedure. |
| Databáze: | OpenAIRE |
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