Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity
| Autor: | Tomomi Watanabe-Asaka, Nariaki Arai, Ryo Kajihara, Toshio Ohhashi, Yumiko Yokoyama, Daisuke Maejima, Moyuru Hayashi, Maki Kaidoh, Yoshiko Kawai, Kei Amari, Sachiho Nagashio |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Physiology Drinking Stimulation Interleukin 22 03 medical and health sciences Adenosine Triphosphate 0302 clinical medicine Downregulation and upregulation Physiology (medical) Intestine Small medicine Humans Lymphocytes Intestinal Mucosa Myofibroblasts Immunity Mucosal Lamina propria Membrane Glycoproteins Hepatology ATP synthase biology Chemistry Innate lymphoid cell Gastroenterology Immunity Innate Cell biology 030104 developmental biology medicine.anatomical_structure Podoplanin biology.protein Myofibroblast 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 320:G54-G65 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.00303.2020 |
| Popis: | We previously demonstrated that water intake increased mesenteric lymph flow and the total flux of IL-22 in rat jejunum. The drained water and the higher permeability of albumin in the jejunal microcirculation contributed to increase the lymph flow and IL-22 transport via the activation of great bulk flow in the jejunal villi. To address the effects of water intake-mediated great bulk flow-dependent mechanical force on jejunal physiological function and immunological regulation of innate lymphoid cells (ILC)-3, we examined the effects of shear stress stimulation on cultured rat myofibroblast cells. Next, we investigated the effects of water intake on podoplanin and IL-22 expressions in cultured human intestinal epithelial cells and rat in vivo jejunal preparations, respectively. Shear stress stimulation of the myofibroblast cells induced ATP release via an activation of cell surface F1/F0 ATP synthase. ATP produced podoplanin expression in the intestinal epithelial cells. Water intake accelerated immunohistochemical expressions of podoplanin and IL-22 in the interepithelial layers and lamina propria of the jejunum. ATP dose-dependently increased IL-22 mRNA expression in ILC-3, which are housed in the lamina propria. Water intake also increased immunohistochemical and mRNA expressions of ecto-nucleoside triphosphate diphosphohydrolases 2 and 5 in jejunal villi. In conclusion, water intake-mediated shear stress stimulation-dependent ATP release from myofibroblast cells maintains higher tissue colloid osmotic pressure in the jejunal microcirculation through podoplanin upregulation in the interepithelial layers. ATP induces IL-22 mRNA expression in ILC-3 in jejunal villi, which may contribute to regulation of mucosal immunity in small intestine.NEW & NOTEWORTHY We investigated effects of shear stress stimulation on cultured myofibroblast cells and water intake on podoplanin and IL-22 expressions in rat jejunal villi. The stimulation induced ATP release from the cells. Water intake accelerated podoplanin and IL-22 expression levels. ATP increased IL-22 mRNA expression in innate lymphoid cells (ILC)-3. Hence, water intake maintains higher osmotic pressure in the jejunal villi through ATP release and podoplanin upregulation. Water intake may regulate the mucosal immunity. |
| Databáze: | OpenAIRE |
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