A new immunosensor for breast cancer cell detection using antibody-coated long alkylsilane self-assembled monolayers in a parallel plate flow chamber

Autor: Jean-Claude Ehrhart, Arnaud Brosseau, B. Bennetau, Phuong Lan Tran, Jean-Pierre Madrange, Louis Renaud, Patrick Tauc, Sylvain Allano, Laurent Thomas, Julie Morisot
Přispěvatelé: Institut des Sciences Moléculaires (ISM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Centre de recherche cerveau et cognition (CERCO), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Physics, University of Wales, Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires (PPSM), École normale supérieure - Cachan (ENS Cachan)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2008
Předmět:
MESH: Cell Line
Tumor
Parallel-plate flow chamber
Biomedical Engineering
Biophysics
MESH: Antigens
Neoplasm
Breast Neoplasms
Nanotechnology
Biosensing Techniques
02 engineering and technology
MESH: Antibodies
Monoclonal
Antigen-Antibody Reactions
03 medical and health sciences
chemistry.chemical_compound
Antigens
Neoplasm
Trichlorosilane
Cell Line
Tumor
Monolayer
Electrochemistry
MESH: Antigen-Antibody Reactions
Humans
MESH: Silanes
Alkyl
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
MESH: Humans
Antibodies
Monoclonal
Laminar flow
Self-assembled monolayer
General Medicine
Silanes
021001 nanoscience & nanotechnology
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
chemistry
Chemical engineering
Triethoxysilane
MESH: Cell Adhesion Molecules
Female
0210 nano-technology
MESH: Female
Cell Adhesion Molecules
Biosensor
MESH: Biosensing Techniques
MESH: Breast Neoplasms
Biotechnology
Zdroj: Biosensors and Bioelectronics
Biosensors and Bioelectronics, Elsevier, 2008, 24 (3), pp.467-74. ⟨10.1016/j.bios.2008.04.027⟩
ISSN: 0956-5663
Popis: International audience; We designed a new efficient and reliable immunosensor and demonstrated its analytic performance to capture breast cancer MCF7 and T47D cells, under laminar flow, onto antibody-coated long alkylsilane self-assembled monolayers (SAMs) in a parallel plate flow chamber. The surface floor of the laminar flow chamber was grafted with an amino-terminated long alkyl chain spacer, 21-aminohenicosyl trichlorosilane (AHTS) followed by tethering a specific monoclonal antibody directed against the human epithelial cell adhesion molecule (EpCAM) antigen, which is overexpressed in primary breast cancer. Properties of the AHTS- and antibody-grafted surface floor were compared to that of surface floors coated with the short alkyl spacers 3-glycidoxy-propyl trimethoxysilane (GPTS) or 3-aminopropyl triethoxysilane (APTES) and antibodies. A theoretical model was constructed according to the geometry of the flow chamber in order to calculate the trajectories that would use cell flows. Cell capture experiments demonstrated that cell immobilization was optimized throughout the whole flow chamber. High cell capture was yielded on antibody-tethered long alkyl AHTS surface. This new procedure offers multiple advantages: a versatile tool readily applied to a panel of purified antibodies, an enrichment of cell immobilization using repetitive cell flow, and a stable capturing surface suitable for long term storage and handling.
Databáze: OpenAIRE
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