UVA, UVB Light Doses and Harvesting Time Differentially Tailor Glucosinolate and Phenolic Profiles in Broccoli Sprouts
| Autor: | Luis Cisneros-Zevallos, Vimal Nair, Melissa Moreira-Rodríguez, Daniel A. Jacobo-Velázquez, Jorge Benavides |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Time Factors abiotic stress Ultraviolet Rays Glucosinolates glucosinolate profiles Pharmaceutical Science Brassica UVA UVB light 01 natural sciences Article UV radiation Analytical Chemistry lcsh:QD241-441 Glucobrassicin 03 medical and health sciences chemistry.chemical_compound Phenols lcsh:Organic chemistry Functional Food Drug Discovery Botany Ultraviolet light Food science Gallic acid Physical and Theoretical Chemistry broccoli Glucoraphanin Ethanol Molecular Structure Organic Chemistry sprouts phenolic profiles 030104 developmental biology chemistry Seedlings Chemistry (miscellaneous) Glucosinolate Molecular Medicine Broccoli sprouts Methanol 010606 plant biology & botany |
| Zdroj: | Molecules; Volume 22; Issue 7; Pages: 1065 Molecules, Vol 22, Iss 7, p 1065 (2017) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules22071065 |
| Popis: | Broccoli sprouts contain health-promoting glucosinolate and phenolic compounds that can be enhanced by applying ultraviolet light (UV). Here, the effect of UVA or UVB radiation on glucosinolate and phenolic profiles was assessed in broccoli sprouts. Sprouts were exposed for 120 min to low intensity and high intensity UVA (UVAL, UVAH) or UVB (UVBL, UVBH) with UV intensity values of 3.16, 4.05, 2.28 and 3.34 W/m2, respectively. Harvest occurred 2 or 24 h post-treatment; and methanol/water or ethanol/water (70%, v/v) extracts were prepared. Seven glucosinolates and 22 phenolics were identified. Ethanol extracts showed higher levels of certain glucosinolates such as glucoraphanin, whereas methanol extracts showed slight higher levels of phenolics. The highest glucosinolate accumulation occurred 24 h after UVBH treatment, increasing 4-methoxy-glucobrassicin, glucobrassicin and glucoraphanin by ~170, 78 and 73%, respectively. Furthermore, UVAL radiation and harvest 2 h afterwards accumulated gallic acid hexoside I (~14%), 4-O-caffeoylquinic acid (~42%), gallic acid derivative (~48%) and 1-sinapoyl-2,2-diferulolyl-gentiobiose (~61%). Increases in sinapoyl malate (~12%), gallotannic acid (~48%) and 5-sinapoyl-quinic acid (~121%) were observed with UVBH Results indicate that UV-irradiated broccoli sprouts could be exploited as a functional food for fresh consumption or as a source of bioactive phytochemicals with potential industrial applications. |
| Databáze: | OpenAIRE |
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