D-2-Hydroxyglutarate producing neo-enzymatic activity inversely correlates with frequency of the type of isocitrate dehydrogenase 1 mutations found in glioma
| Autor: | Stefan Pusch, Aubry K. Miller, Jörg Balss, Leonille Schweizer, Ann Christin Beck, Susanne Weissert, Johanna Marie Lehmler, Andreas von Deimling |
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| Jazyk: | angličtina |
| Předmět: |
Pathology
medicine.medical_specialty IDH1 Cell Survival Green Fluorescent Proteins Oligodendroglioma Mutant Astrocytoma Transfection Pathology and Forensic Medicine Glutarates D-2-hydroxyglutarate Cellular and Molecular Neuroscience Cell Movement Mutant protein Glioma medicine Humans Tumor Stem Cell Assay chemistry.chemical_classification Enzymatic activity Brain Neoplasms business.industry Research Wild type 2-HG medicine.disease Molecular biology Isocitrate Dehydrogenase KM HEK293 Cells Enzyme Isocitrate dehydrogenase chemistry Mutagenesis Cell culture Mutation Mutant Proteins Neurology (clinical) business |
| Zdroj: | Acta Neuropathologica Communications |
| ISSN: | 2051-5960 |
| DOI: | 10.1186/2051-5960-2-19 |
| Popis: | Background IDH mutations frequently occur in diffuse gliomas and result in a neo-enzymatic activity that results in reduction of α-ketoglutarate to D-2-hydroxyglutarate. In gliomas, the frequency of IDH1 mutations in codon 132 increases in the order R132L-R132S-R132G-R132C-R132H with R132H constituting more than 90% of all IDH1 mutations. Results We determined the levels of D-2-hydroxyglutarate in glioma tissues with IDH1 mutations. D-2-hydroxyglutarate levels increased in the order of R132H-R132C-R132S/R132G/R132L. We expressed and purified IDH1 wild type and mutant protein for biochemical characterization. Enzyme kinetics of mutant IDH protein correlated well with D-2-hydroxyglutarate production in cells with R132H exhibiting the highest and R132L the lowest KM for α-ketoglutarate. Addition of D-2-hydroxyglutarate to the medium of cell lines revealed an inhibitory effect at higher concentrations. Migration of LN229 increased at lower D-2-hydroxyglutarate concentrations while higher concentrations showed no effect. Conclusion These findings may suggest natural selection against the rare IDH1R132 mutations in human glioma due to toxicity caused by high levels of D-2-hydroxyglutarate. |
| Databáze: | OpenAIRE |
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