mRNA expression profile of the TNF-α system in LH-induced bovine preovulatory follicles and effects of TNF-α on gene expression, ultrastructure and expansion of cumulus-oocyte complexes cultured in vitro

Autor: Christina Alves Peixoto, M.A.M. Donato, J. R. V. Silva, Werner G. Glanzner, A. M. P. Dau, P. B. D. Gonçalves, JT dos Santos, Vilceu Bordignon, A. W. B. Silva, Gustavo Freitas Ilha, Monique Tomazele Rovani, E.V. Cunha, J.J.N. Costa, F.T.G. Bezerra
Rok vydání: 2017
Předmět:
0301 basic medicine
endocrine system
medicine.medical_specialty
media_common.quotation_subject
medicine.medical_treatment
Gene Expression
Ovary
Biology
Gonadotropin-Releasing Hormone
Andrology
03 medical and health sciences
0302 clinical medicine
Ovarian Follicle
Food Animals
Internal medicine
Gene expression
Follicular phase
medicine
Animals
Receptors
Tumor Necrosis Factor
Type II
RNA
Messenger
Small Animals
Ovulation
Cells
Cultured
media_common
Cumulus Cells
030219 obstetrics & reproductive medicine
Tumor Necrosis Factor-alpha
Equine
Luteinizing Hormone
Oocyte
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Cytokine
Receptors
Tumor Necrosis Factor
Type I
Cell culture
Oocytes
Cattle
Female
Animal Science and Zoology
Tumor necrosis factor alpha
hormones
hormone substitutes
and hormone antagonists
Zdroj: Theriogenology. 90:1-10
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2016.11.015
Popis: This study evaluated (1) the effects of in vivo GnRH treatment on mRNA expression of TNF-α system (TNF-α, TNFR1 and TNFR2) in granulosa cells of bovine preovulatory follicles, (2) the in vitro influence of gonadotropins on mRNA expression of TNF-α system in cultured cumulus cells, (3) the protein expression of the TNF-α system in late antral follicles and, (4) the influence of TNF-α on cumulus cells expansion, ultrastructure and on expression of HAS2, CASP3 and CASP6 in follicular cells cultured for 24 h. An increased expression of TNF-α and TNFR1 was observed after 3, 6 and 12 h of GnRH treatment when compared to 0 and 24h. Higher TNFR2 mRNA levels were observed 3, 6 and 12 h after GnRH, when compared to 0 and 24 h. Proteins of TNF-α system were also expressed in late antral follicles. In vitro, TNF-α did not affect cumulus cells expansion, but reduced the HAS2, CASP3 and CASP6 mRNA levels in cumulus cells after 12 h. After 24 h of culture, TNF-α increased the mRNA levels for CASP6 in mural granulosa cells, while the TNF-α, TNFR1 and TNFR2 mRNA levels were increased in cumulus-oocyte complexes (COCs) cultured for 12 h with gonadotropins, but not after 24 h. Ultrastructural analysis confirmed the integrity of COCs cultured in presence of TNF-α. In conclusion, TNF-α system members are present in bovine antral follicles and expression of TNF-α is influenced by gonadotropins in vivo and in vitro. In vitro, TNF-α maintained cumulus cells ultrastructure during COC culture.
Databáze: OpenAIRE
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