A Formulated TLR7/8 Agonist is a Flexible, Highly Potent and Effective Adjuvant for Pandemic Influenza Vaccines
| Autor: | Sarah C Evans, Neal Van Hoeven, Brian Granger, James Elvecrog, Rie Nakajima, Darrick Carter, Nicole L. Marlenee, Li Liang, Ghislain I. Nana, Hong Liang, Sharvari W Joshi, Tara Evers, Susan Lin, Rhea N. Coler, Christopher B. Fox, Philip L. Felgner, Susan L. Baldwin, Airn E. Hartwig, Richard A. Bowen, Mark A. Tomai, Steven G. Reed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
and promotion of well-being medicine.medical_treatment medicine.disease_cause chemistry.chemical_compound 0302 clinical medicine Immunologic Influenza A virus Influenza A Virus 030212 general & internal medicine Multidisciplinary virus diseases Infectious Diseases 3.4 Vaccines Influenza Vaccines 5.1 Pharmaceuticals Pneumonia & Influenza H5N1 Subtype Development of treatments and therapeutic interventions Infection Adjuvant Human Agonist H5N1 vaccine medicine.drug_class Hemagglutinin (influenza) Biology Virus Article Vaccine Related 03 medical and health sciences Adjuvants Immunologic Biodefense Influenza Human medicine Humans Adjuvants Influenza A Virus H5N1 Subtype Prevention Prevention of disease and conditions Virology Influenza A virus subtype H5N1 Influenza Imidazoquinoline 030104 developmental biology Emerging Infectious Diseases Good Health and Well Being chemistry Toll-Like Receptor 7 Toll-Like Receptor 8 biology.protein Immunization |
| Zdroj: | Scientific reports, vol 7, iss 1 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep46426 |
| Popis: | Since 1997, highly pathogenic avian influenza viruses of the H5N1 subtype have been transmitted from avian hosts to humans. The severity of H5N1 infection in humans, as well as the sporadic nature of H5N1 outbreaks, both geographically and temporally, make generation of an effective vaccine a global public health priority. An effective H5N1 vaccine must ultimately provide protection against viruses from diverse clades. Toll-like receptor (TLR) agonist adjuvant formulations have a demonstrated ability to broaden H5N1 vaccine responses in pre-clinical models. However, many of these agonist molecules have proven difficult to develop clinically. Here, we describe comprehensive adjuvant formulation development of the imidazoquinoline TLR-7/8 agonist 3M-052, in combination with H5N1 hemagglutinin (HA) based antigens. We find that 3M-052 in multiple formulations protects both mice and ferrets from lethal H5N1 homologous virus challenge. Furthermore, we conclusively demonstrate the ability of 3M-052 adjuvant formulations to broaden responses to H5N1 HA based antigens, and show that this broadening is functional using a heterologous lethal virus challenge in ferrets. Given the extensive clinical use of imidazoquinoline TLR agonists for other indications, these studies identify multiple adjuvant formulations which may be rapidly advanced into clinical trials in an H5N1 vaccine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|