Gene duplication in haloaromatic degradative plasmids pJP4 and pJP2
| Autor: | Debabrota Ghosal, In-Soon You |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
DNA
Bacterial Immunology Biology Applied Microbiology and Biotechnology Microbiology Gene dosage DNA sequencing Homology (biology) chemistry.chemical_compound Plasmid Pseudomonas Sequence Homology Nucleic Acid Gene duplication Genetics Alcaligenes Cloning Molecular Molecular Biology Genomic organization Electrophoresis Agar Gel General Medicine Blotting Southern Microscopy Electron chemistry Multigene Family Transformation Bacterial Tandem exon duplication DNA Plasmids |
| Zdroj: | Canadian Journal of Microbiology. 34:709-715 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m88-121 |
| Popis: | pJP2 and pJP4 are 2,4-dichlorophenoxyacetic acid catabolic plasmids, and they show DNA sequence homology. Most of the pJP2 molecules (80% or more) isolated from 2,4-dichlorophenoxyacetic acid grown cells of Alcaligenes eutrophus harbor a tandem duplication of a 25-kilobase (kb) segment encoding the catabolic functions. Unlike plasmid pJP4, pJP2 in A. eutrophus gives rise to a 3-chlorobenzoate phenotype without further genetic rearrangement. pJP4 under 3-chlorobenzoate selection contains an inverted duplication of 24.5 kb. Absence of selective pressure results in the prompt loss of one copy of the duplication in pJP4, but not of the tandem duplication in pJP2. In both pJP4 and pJP2, mutation of the duplicated copy, rather than gene dosage, is likely to be the basis of phenotypic change of catabolic functions. Experiments using the cloned DNA suggest that a tandem duplication is more stable than an inverted duplication. |
| Databáze: | OpenAIRE |
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