IncX4 Plasmid Carrying the New mcr-1.9 Gene Variant in a CTX-M-8-Producing Escherichia coli Isolate Recovered From Swine
| Autor: | Eugénia Ferreira, Catarina Oliveira Silva, Manuela Caniça, Luís Vieira, Lurdes Clemente, Vera Manageiro, Raquel Romão |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
lcsh:QR1-502 Biology medicine.disease_cause Microbiology Genome lcsh:Microbiology CTX-M-8 03 medical and health sciences Plasmid IncX4 Escherichia coli medicine Replicon Gene 030304 developmental biology 2. Zero hunger 0303 health sciences Portugal Resistência aos Antimicrobianos 030306 microbiology 3. Good health Colistin MCR-1 Mobile genetic elements Plasmid-mediated Colistin Resistance MCR-1.9 hormones hormone substitutes and hormone antagonists plasmid-mediated colistin resistance medicine.drug |
| Zdroj: | Frontiers in Microbiology, Vol 10 (2019) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2019.00367 |
| Popis: | We studied a commensal colistin-resistant Escherichia coli isolated from a swine cecum sample collected at a slaughter, in Portugal. Antimicrobial susceptibility phenotype of E. coli LV23529 showed resistance to colistin at a minimum inhibitory concentration of 4 mg/L. Whole genome of E. coli LV23529 was sequenced using a MiSeq system and the assembled contigs were analyzed for the presence of antibiotic resistance and plasmid replicon types using bioinformatics tools. We report a novel mcr-1 gene variant (mcr-1.9), carried by an IncX4 plasmid, where one-point mutation at nucleotide T1238C leads to Val413Ala substitution. The mcr-1.9 genetic context was characterized by an IS26 element upstream of the mcr-pap2 element and by the absence of ISApl1. Bioinformatic analysis also revealed genes conferring resistance to β-lactams, sulphamethoxazole, trimethoprim, chloramphenicol and colistin, corresponding to the phenotype noticed. Moreover, we highlight the presence of mcr-1.9 plus blaCTX-M-8, a blaESBL gene rarely detected in Europe in isolates of animal origin; these two genes were located on different plasmids with 33,303 and 89,458 bp, respectively. MCR-1.9-harboring plasmid showed high identity to other X4-type mcr-1-harboring plasmids characterized worldwide, which strongly suggests that the presence of PMCR-encoding genes in food-producing animals, such as MCR-1.9, represent a potential threat to humans, as it is located in mobile genetic elements that have the potential to spread horizontally. VM was supported by Fundação para a Ciência e a Tecnologia (FCT) fellowship (Grant No. SFRH/BPD/77486/2011), financed by the European Social Funds (COMPETE-FEDER), and National Funds of the Portuguese Ministry of Education and Science (POPH-QREN). The authors thank to FCT for the project grant UID/MULTI/00211/2013. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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