One potential oncolytic adenovirus expressing Lipocalin-2 for colorectal cancer therapy
| Autor: | Jin-Feng Feng, Bin Xu, Hai-yan Ge, Xiu-Yan Huang, Wen-Yan Zheng |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Oncolytic adenovirus
Oncology Cancer Research medicine.medical_specialty Colorectal cancer Genetic enhancement Blotting Western Genetic Vectors Mice Nude Apoptosis Malignancy Virus Replication Adenoviridae Immunoenzyme Techniques Mice Nude mouse Lipocalin-2 Internal medicine Proto-Oncogene Proteins medicine Tumor Cells Cultured Animals Humans Radiology Nuclear Medicine and imaging Cell Proliferation Pharmacology Oncolytic Virotherapy integumentary system biology Cell growth business.industry General Medicine Genetic Therapy medicine.disease biology.organism_classification Flow Cytometry Combined Modality Therapy Xenograft Model Antitumor Assays Lipocalins Cytolysis business Colorectal Neoplasms Acute-Phase Proteins |
| Zdroj: | Cancer biotherapyradiopharmaceuticals. 28(5) |
| ISSN: | 1557-8852 |
| Popis: | Colorectal cancer is an aggressive malignancy with a high mortality rate; however, effective therapies are currently lacking. Cancer-targeting gene-virotherapy (CTGVT) has been proposed to be a promising strategy for cancer therapy. The purpose of this study was to investigate the antitumor activity of the oncolytic adenovirus harboring Lipocalin-2 (ZD55-Lipocalin-2, an example of CTGVT) in colorectal cancer. ZD55-Lipocalin-2 was generated by deleting E1B55-KD and inserting the Lipocalin-2 gene. Its cytopathic effects and cell growth inhibition were detected in vitro, and antitumor effects were examined in a nude mouse model of human colorectal cancer xenografts. Results showed that ZD55-Lipocalin-2 significantly inhibited the colorectal cancer growth by selective cytolysis, inducing apoptosis and decreasing the microvessel density in tumors. The anticancer potential of ZD55-Lipocalin-2 showed stronger than that of the isolated Lipocalin-2 gene therapy or isolated ZD55 oncolytic adenovirus therapy. ZD55-Lipocalin-2 may serve as a potential anticancer agent for colorectal cancer treatment. |
| Databáze: | OpenAIRE |
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